Influence of gut microbiota on mouse B2 B cell ontogeny and function
Influence of gut microbiota on mouse B2 B cell ontogeny and function
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DOI：
10.1016/j.molimm.2011.02.002
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发表时间：
2011-05-01
影响因子：
3.6
通讯作者：
Benyacoub, Jalil
中科院分区：
文献类型：
--
作者：
Hansson, Jenny;Bosco, Nabil;Benyacoub, Jalil
微生物群和宿主免疫系统之间的复杂相互作用被证明可以在整个生命过程中塑造免疫系统，但关于微生物对适应性免疫系统的关键参与者B2 B细胞的影响知之甚少。在本研究中，我们评估了肠道细菌对B细胞个体发育和功能的影响，重点是脾脏和派伊尔集合淋巴结的B2 B细胞。我们将无菌小鼠与从出生之日起暴露于正常复杂细菌群落的小鼠进行了比较，并将经典免疫学评估与先进的全基因组表达谱分析相结合。尽管保留了所有B细胞亚群和表型，但我们的结果显示微生物群强烈影响粘膜B细胞生理学并导致较高的血清IG浓度。我们表明，这种微生物的影响包括下调转录因子参与早期B细胞活化步骤和上调基因和蛋白质参与后期阶段的B细胞反应。总之，我们显示了肠道微生物群对粘膜B2 B细胞功能的影响，涉及B细胞活化和增殖的下游机制。(C)2011爱思唯尔有限公司保留所有权利。
A complex interplay between the microbiota and the host immune system is evidenced to shape the immune system throughout life, but little is known about the microbial effect on key players of the adaptive immune system, the B2 B cells. In the presented study, we have evaluated the effect of commensal bacteria on B cell ontogeny and function, with the focus on B2 B cells of spleen and Peyer's patches. We have compared germ-free mice to mice that are exposed to a normal complex bacterial community from the day of birth and combined classical immunological assessment with advanced genome-wide expression profiling. Despite a preservation of all B cell subsets and phenotype, our results show that microbiota strongly impact mucosal B cell physiology and lead to higher serum Ig concentrations. We show that this microbial influence comprises downregulation of transcription factors involved in early B cell activation steps and upregulation of genes and proteins involved in later stages of B cell response. In summary, we show an influence of the gut microbiota on function of mucosal B2 B cells, involving mechanisms downstream of B cell activation and proliferation. (C) 2011 Elsevier Ltd. All rights reserved.