Narrowband ultraviolet B radiation suppresses contact hypersensitivity
Narrowband ultraviolet B radiation suppresses contact hypersensitivity
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DOI：
10.1111/j.1600-0781.2008.00333.x
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发表时间：
2008-02-01
影响因子：
2.6
通讯作者：
Morita, Akimichi
中科院分区：
文献类型：
--
作者：
Shintani, Yoichi;Yasuda, Yoko;Morita, Akimichi
背景/目的：窄带紫外线（UV）B的功效的主要机制被认为是诱导病理相关细胞的凋亡。然而，窄带UVB治疗通常诱导相对较长的缓解期。最近，UVB辐射诱导调节性T（Treg）细胞的证据被报道。基于这些发现，我们研究了窄带UVB是否通过诱导Treg细胞来抑制接触性超敏反应（CHS）。方法：用宽波段或窄波段UVB照射C3 H/HeN小鼠的毛细胞。CHS定义为耳肿胀反应。为了检查是否可以通过过继转移诱导耐受，在致敏和CHS攻击之前将来自UVB照射小鼠的淋巴结细胞注射到幼稚小鼠中。结果：窄谱UVB照射剂量依赖性地抑制CHS。在1000 ~ 3000 mJ/cm（2）剂量范围内，对细胞增殖有显著抑制作用（P < 0.05）。用1000 mJ/cm（2）窄带UVB实现的抑制效果与用100 mJ/cm（2）宽带UVB实现的效果非常相似。通过注射耐受小鼠的淋巴结细胞，将对CHS的抑制作用转移到幼稚小鼠。结论：窄谱UVB对CHS有局部和全身抑制作用。此外，窄谱UVB诱导对CHS的耐受，并且抑制效应可转移到命名小鼠。
Background/ purpose: A main mechanism responsible for the efficacy of narrowband ultraviolet (UV)B is thought to be the induction of apoptosis in pathogenetically relevant cells. Narrowband UVB therapy, however, generally induces a relatively long remission period. Recently; evidence that UVB radiation induces regulatory T (Treg) cells was reported. Based on these findings, we examined whether narrowband UVB suppresses contact hypersensitivity (CHS) by inducing Treg cells. Methods: The shaved abdomens of C3H/HeN mice were irradiated with broadband or narrowband UVB. CHS was defined as an ear-swelling response. To examine whether tolerance can be induced by adoptive transfer, lymph node cells from UVB-irradiated mice were injected into naive mice before sensitization and CHS challenge. Results: Narrowband UVB exposure dose dependently suppressed CHS. Significant suppression was observed at doses between 1000 and 3000 mJ/cm(2) (P < 0.05). The suppressive effect achieved with 1000 mJ/cm(2) narrowband UVB was very similar to the effect achieved with 100 mJ/cm(2) broadband UVB. The suppressive effects on CHS were transferred to naive mice by the injection of lymph node cells from tolerant mice. Conclusion: Narrowband UVB induced local and systemic suppression of CHS. In addition, narrowband UVB induces tolerance to CHS and the suppressive effects were transferable to name mice.