The Vibrio parahaemolyticus pvuA1 gene (formerly termed psuA) encodes a second ferric vibrioferrin receptor that requires tonB2.
The Vibrio parahaemolyticus pvuA1 gene (formerly termed psuA) encodes a second ferric vibrioferrin receptor that requires tonB2.
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DOI：
10.1111/j.1574-6968.2011.02389.x
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发表时间：
2011-11
影响因子：
2.1
通讯作者：
T. Tanabe;Tatsuya Funahashi;Noriyuki Okajima;H. Nakao;Y. Takeuchi;K. Miyamoto;H. Tsujibo;Shigeo Yamamoto
中科院分区：
文献类型：
--
作者：
T. Tanabe;Tatsuya Funahashi;Noriyuki Okajima;H. Nakao;Y. Takeuchi;K. Miyamoto;H. Tsujibo;Shigeo Yamamoto
我们以前报道，副溶血性弧菌pvsABCDE和psuA-pvuABCDE操纵子参与其自身的铁载体，弧菌铁蛋白（VF）的生物合成和运输。其中，psuA和pvuA编码依赖于TonB的外膜蛋白（OMP）。虽然pvuA的特点是铁弧菌铁蛋白受体基因，psuA产品的作用仍然未知。在这项研究中，生长试验的同基因psuA，pvuA，和psuA-pvuA双缺失突变体，然后与psuA或pvuA的双缺失突变体的互补被用来确定psuA作为一个基因编码的OMP参与铁VF的摄取。因此，psuA和pvuA分别被重命名为pvuA 1和pvuA 2。此外，我们阐明了PvuA 1和PvuA 2的TonB特异性，因为副溶血性弧菌有三套TonB系统。pvuA 1、tonB 1和tonB 2的三重缺失以及pvuA 2和tonB 2的双重缺失导致VF对生长的促进作用完全丧失。这一发现表明PvuA 1和PvuA 2运输铁VF穿过外膜所需的能量分别由TonB 2系统以及TonB 1和TonB 2系统提供。
We previously reported that the Vibrio parahaemolyticus pvsABCDE and psuA-pvuABCDE operons are involved in the biosynthesis and transport of its own siderophore, vibrioferrin (VF). Of these, psuA and pvuA encode TonB-dependent outer-membrane proteins (OMPs). Although pvuA was characterized as the ferric vibrioferrin receptor gene, the role of the psuA product remains unknown. In this study, a growth assay of isogenic psuA, pvuA, and psuA-pvuA double-deletion mutants followed by complementation of the double-deletion mutant with psuA or pvuA was used to identify psuA as a gene encoding an OMP involved in the uptake of ferric VF. Thus, psuA and pvuA were renamed pvuA1 and pvuA2, respectively. Moreover, we clarified the TonB specificities of PvuA1 and PvuA2, because V. parahaemolyticus has three sets of the TonB systems. The triple deletion of pvuA1, tonB1, and tonB2, and the double deletion of pvuA2 and tonB2 resulted in the complete loss of growth promotion by VF. This finding indicates that the energy required for PvuA1 and PvuA2 to transport ferric VF across the outer membrane is provided by the TonB2 system and by both the TonB1 and TonB2 systems, respectively.