Peroral infectivity of non-occluded viruses of Bombyx mori nucleopolyhedrovirus and polyhedrin-negative recombinant baculoviruses to silkworm larvae is drastically enhanced when administered with Anomala cuprea entomopoxvirus spindles.
Peroral infectivity of non-occluded viruses of Bombyx mori nucleopolyhedrovirus and polyhedrin-negative recombinant baculoviruses to silkworm larvae is drastically enhanced when administered with Anomala cuprea entomopoxvirus spindles.
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当与Anomala cuprea昆虫痘病毒纺锤体一起施用时，家蚕核多角体病毒和多角体蛋白阴性重组杆状病毒的非包涵体病毒对家蚕幼虫的经口感染性显着增强。
DOI：
10.1099/0022-1317-82-2-307
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
M. Sato
中科院分区：
文献类型：
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作者：
Y. Furuta;W. Mitsuhashi;J. Kobayashi;S. Hayasaka;S. Imanishi;Y. Chinzei;M. Sato
家蚕核型多角体病毒（BmNPV）的非封闭型病毒（NOV）经口接种对家蚕幼虫的感染性很差，但注射到昆虫血腔中具有很强的感染性。在本研究中，它表明，NOVs的BmNPV成为高度感染性，即使通过口服接种时，与纺锤体（蛋白质结构）的Anomala cuprea昆虫痘病毒（AcEPV）。在测试的家蚕幼虫的所有生长阶段（第2至第5龄）中观察到AcEPV纺锤体对NOV经口感染性的显著增强（在最强的情况下高近1000倍）。同样，多角体蛋白阴性重组体的BmNPV，不产生多角体，经口感染性也显着增强的AcEPV纺锤体。与此相反，球状体（蛋白质结构，含有AcEPV病毒粒子）并没有提高经口感染的任何NOV或重组家蚕核型多角体病毒在家蚕幼虫。
Non-occluded viruses (NOVs) of Bombyx mori nucleopolyhedrovirus (BmNPV) are poorly infectious to silkworm larvae when administered by peroral inoculation, although they are highly infectious when injected into the insect haemocoel. In the present study, it is demonstrated that NOVs of BmNPV became highly infectious even through peroral inoculation when administered with spindles (proteinaceous structures) of Anomala cuprea entomopoxvirus (AcEPV). Marked enhancement of peroral infectivity of NOVs by AcEPV spindles (nearly 1000-fold higher in the strongest case) was observed in all growth stages of silkworm larvae tested (2nd to 5th instar). Similarly, peroral infectivity of polyhedrin-negative recombinants of BmNPV, which do not produce polyhedra, was also enhanced remarkably by AcEPV spindles. In contrast, spheroids (proteinaceous structures containing AcEPV virions) did not enhance the peroral infectivity of either NOVs or the recombinant BmNPV in silkworm larvae.
Kenxin Sun、Masahiro Yamaguchi、Masao Yuda、Ken Miura、Hiroyuki Takeya、Makoto Hirai、Hiroyuki Masuoka、Katsuhiko Ando、Takashi Watanabe、Koji Suzuki 和 Yasuo Chinzei：“一种新型抗凝血血红素蛋白的纯化、表征和 cDNA 克隆
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