The Murine Femoral Bone Graft Model and a Semiautomated Histomorphometric Analysis Tool
The Murine Femoral Bone Graft Model and a Semiautomated Histomorphometric Analysis Tool
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DOI：
10.1007/978-1-62703-989-5_4
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发表时间：
2014-01-01
期刊：
影响因子：
--
通讯作者：
Xie, Chao
中科院分区：
文献类型：
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作者：
Dhillon, Robinder S.;Zhang, Longze;Xie, Chao
骨修复的临床前研究仍然是一个高度优先，因为尚未解决的临床问题与治疗关键节段性缺损和骨折愈合的并发症。在过去的十年中，小鼠股骨同种异体移植模型已经得到普及，由于其标准化的手术和检查的放射学，生物力学和组织学结果的措施，大量的潜力。在这里，我们描述了这些方法和一种新的半自动组织形态计量学方法来量化的量的骨，软骨，和未分化的间充质组织在脱矿石蜡切片同种异体移植的小鼠股骨使用Visiopharm图像分析软件系统。
Preclinical studies of bone repair remain a high priority because of unresolved clinical problems associated with treating critical segmental defects and complications of fracture healing. Over the last decade, the murine femoral allograft model has gained popularity due to its standardized surgery and potential for examining a vast array of radiographic, biomechanical, and histological outcome measures. Here, we describe these methods and a novel semiautomated histomorphometric approach to quantify the amount of bone, cartilage, and undifferentiated mesenchymal tissue in demineralized paraffin sections of allografted murine femurs using the Visiopharm Image Analysis Software System.