Study on the effects of gradient mechanical pressures on the proliferation, apoptosis, chondrogenesis and hypertrophy of mandibular condylar chondrocytes in vitro
Study on the effects of gradient mechanical pressures on the proliferation, apoptosis, chondrogenesis and hypertrophy of mandibular condylar chondrocytes in vitro
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DOI：
10.1016/j.archoralbio.2016.10.017
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发表时间：
2017-01-01
影响因子：
3
通讯作者：
Zhang, Min
中科院分区：
文献类型：
--
作者：
Li, Hui;Huang, Linjian;Zhang, Min
目的：方法：体外培养5只兔下颌髁突软骨细胞，分别施加50 kPa、100 kPa、150 kPa和200 kPa的静态压力3 h，观察不同压力梯度对软骨细胞增殖、凋亡及软骨形成和肥大标志物表达的影响。无压力（0 kPa）培养的软骨细胞作为对照。观察细胞增殖、凋亡及聚集蛋白聚糖（AGG）、Ⅱ型胶原（COL 2）、X型胶原（COL 10）、碱性磷酸酶（ALP）的表达。结果：100 kPa压力下软骨细胞增殖明显增强，200 kPa压力下软骨细胞增殖明显减弱。软骨细胞凋亡在200 kPa时随峰值压力的增加呈剂量依赖性增加。200 kPa时软骨细胞坏死增加。200 kPa时AGG表达增加。COL 2在50 kPa时表达减少，在150 kPa时表达增加。150 kPa时，COL 10和ALP表达增加。透射电镜观察结果显示：100 kPa时，细胞体积增大，细胞微绒毛减少，细胞核增大; 200 kPa时，细胞体积缩小，出现凋亡细胞。结论：150 kPa的机械负荷是软骨细胞的中等压力。细胞增殖和凋亡平衡，坏死减少，软骨形成和软骨细胞肥大得到促进。当压力较低时，软骨形成和软骨细胞肥大受到抑制。在200 kPa时，暗示软骨退化。(C)2016爱思唯尔有限公司版权所有
Objective: To investigate the effects of gradient mechanical pressure on chondrocyte proliferation, apoptosis, and the expression of markers of chondrogenesis and chondrocyte hypertrophy.Methods: Mandibular condylar chondrocytes from 5 rabbits were cultured in vitro, and pressed with static pressures of 50 kPa, 100 kPa, 150 kPa and 200 kPa for 3 h, respectively. The chondrocytes cultured without pressure (0 kPa) were used as control. Cell proliferation, apoptosis, and the expression of aggrecan (AGG), collagen II (COL2), collagen X (COL10), alkaline phosphatase (ALP) were investigated. Ultrastructures of the pressurized chondrocytes under transmission electron microscopy (TEM) were observed.Results: Chondrocyte proliferation increased at 100 kPa and decreased at 200 kPa. Chondrocyte apoptosis increased with peak pressure at 200 kPa in a dose-dependent manner. Chondrocyte necrosis increased at 200 kPa. The expression of AGG increased at 200 kPa. The expression of COL2 decreased at 50 kPa and increased at 150 kPa. The expression of COL10 and ALP increased at 150 kPa. Ultrastructure of the pressurized chondrocytes under TEM showed: at 100 kPa, cells were enlarged with less cellular microvillus and a bigger nucleus; at 200 kPa, cells shrank with the sign of apoptosis, and apoptosis cells were found.Conclusions: The mechanical loading of 150 kPa is the moderate pressure for chondrocyte: cell proliferation and apoptosis is balanced, necrosis is reduced, and chondrogenesis and chondrocyte hypertrophy are promoted. When the pressure is lower, chondrogenesis and chondrocyte hypertrophy are inhibited. At 200 kPa, degeneration of cartilage is implied. (C) 2016 Elsevier Ltd. All rights reserved.