A highly efficacious lymphocyte chemoattractant, stromal cell-derived factor 1 (SDF-1).
A highly efficacious lymphocyte chemoattractant, stromal cell-derived factor 1 (SDF-1).
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DOI：
10.1084/jem.184.3.1101
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发表时间：
1996-09-01
期刊：
影响因子：
--
通讯作者：
Springer TA
中科院分区：
文献类型：
--
作者：
Bleul CC;Fuhlbrigge RC;Casasnovas JM;Aiuti A;Springer TA
趋化因子被假定为引导淋巴细胞从血流中迁移到炎症部位。趋化性细胞因子家族的成员，称为趋化因子，已显示吸引淋巴细胞，但功效，即，被吸引的细胞的最大百分比一直很低。我们已经确定了一个非常有效的淋巴细胞趋化活性的小鼠骨髓基质细胞系MS-5的上清液中吸引10倍以上的淋巴细胞在体外比目前描述的淋巴细胞化学引诱剂。这种趋化活性的纯化揭示了基质细胞衍生因子1（SDF-1）的身份。SDF-1在体外作用于淋巴细胞和单核细胞，但不作用于嗜中性粒细胞，并且在体内是高效和高效的单核细胞引诱剂。此外，SDF-1诱导淋巴细胞中的细胞内肌动蛋白聚合，这一过程被认为是细胞运动的先决条件。由于SDF-1在广泛的组织中组成型表达，因此它可能在免疫监视和淋巴细胞和单核细胞的基础外渗中而不是在炎症中起作用。
Chemotactic factors are postulated to direct emigration of lymphocytes from the blood stream into sites of inflammation. Members of a family of chemotactic cytokines, termed chemokines, have been shown to attract lymphocytes but efficacy, i.e., the maximal percentage of attracted cells, has been low. We have identified a highly efficacious lymphocyte chemotactic activity in the supernatants of the murine bone marrow stroma cell line MS-5 which attracts 10-fold more lymphocytes in vitro than currently described lymphocyte chemoattractants. Purification of this chemotactic activity revealed identity to stromal cell-derived factor 1 (SDF-1). SDF-1 acts on lymphocytes and monocytes but not neutrophils in vitro and is both a highly efficacious and highly potent mononuclear cell attractant in vivo. In addition, SDF-1 induces intracellular actin polymerization in lymphocytes, a process that is thought to be a prerequisite for cell motility. Since SDF-1 is expressed constitutively in a broad range of tissues it may have a role in immune surveillance and in basal extravasation of lymphocytes and monocytes rather than in inflammation.