Reservoir-type microcapsules prepared by the solvent exchange method: effect of formulation parameters on microencapsulation of lysozyme.
Reservoir-type microcapsules prepared by the solvent exchange method: effect of formulation parameters on microencapsulation of lysozyme.
复制标题
溶剂交换法制备储库型微胶囊：配方参数对溶菌酶微胶囊化的影响。
DOI：
10.1021/mp050083w
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Park,Kinam
中科院分区：
文献类型：
--
作者：
Park,JaeHyung;Ye,Mingli;Yeo,Yoon;Lee,Woo-Kyoung;Paul,Connie;Park,Kinam
A new microencapsulation technique based on the solvent exchange method was implemented using an ultrasonic atomizer system to encapsulate a protein drug in mild conditions. The reservoir-type microcapsules encapsulating lysozyme as a model protein were prepared by inducing collisions between the aqueous droplets containing lysozyme and the droplets of organic solvent with dissolved poly(lactic acid-co-glycolic acid) (PLGA).The main focus of the study was to examine formulation variables on the size and the encapsulation efficiency of the formed microcapsules. The formulation variables examined were concentrations of mannose in the aqueous cores, NaCl in the aqueous collection medium, and PLGA in organic solvent. The mean diameter of the microcapsules ranged from 40 μm to 100 μm. Smaller microcapsules showed lower encapsulation efficiencies. The resulting microcapsules released native lysozyme in a sustained manner, and the release rate was dependent on the formulation conditions, such as the concentration and molecular weight of the polymer used. The solvent exchange method does not induce lysozyme aggregation and loss of its biological activity. The solvent exchange method, implemented by the ultrasonic atomizer system, provides an effective tool to prepare reservoir-type microcapsules for delivering proteins.Keywords: Microencapsulation; microcapsules; mannose; protein delivery; solvent exchange method; PLGA; osmotic pressure
