Two Types of cDNAs Encoding Proopiomelanocortin of Sockeye Salmon, Oncorhynchus nerka
Two Types of cDNAs Encoding Proopiomelanocortin of Sockeye Salmon, Oncorhynchus nerka
复制标题
红鲑鱼阿黑皮素原的两种 cDNA 编码
作者：
Akiko Okuta;H. Ando;H. Ueda;A. Urano
摘要 为了研究红鲑鱼阿片黑皮质素原 (POMC) 基因表达的调控机制，我们分离并鉴定了编码两种类型红鲑鱼 POMC 的 cDNA，即 ssPOMC-A 和 -B。通过使用虹鳟鱼序列，从红鲑垂体的总 RNA 中扩增出两种类型的 PCR 产物。使用 PCR 产物作为探针，从红鲑垂体 cDNA 文库中获得编码 ssPOMCA 和 ssPOMC-B 的全长 cDNA 克隆。 ssPOMC-A和-B cDNA的长度分别为1072和1709 bp。 Northern blot分析显示ssPOMC-A和-B mRNA均仅在垂体中表达，其大小分别约为1.2 kb和1.8 kb。通过对从单个红鲑鱼获得的基因组 DNA 进行 Southern 印迹分析，证实了两个 ssPOMC 基因的存在。 ssPOMC-A和-B的推导氨基酸序列分别包含230和226个残基。 ssPOMC-B中对应于Met-脑啡肽结构域的β-内啡肽的氨基末端是YSGFM，这与许多其他脊椎动物中发现的Met-脑啡肽的YGGFM不同。 ssPOMC-A和-B之间的核苷酸序列在整个编码区中的同源性为59%，而α-MSH编码区具有高度同源性(91%)。尽管推导的ssPOMC的氨基酸序列显示出43%的总体相似性，但它们的亲水特性与其他几种脊椎动物的一致，特别是N端肽(NPP)的氨基末端显示出与其他脊椎动物NPP几乎相同的模式。
Abstract To investigate regulatory mechanisms of proopiomelanocortin (POMC) gene expression in sockeye salmon, we have isolated and characterized cDNAs encoding two types of sockeye salmon POMC, which are referred to as ssPOMC-A and -B. Two types of PCR products were amplified from total RNA of sockeye salmon pituitaries by use of rainbow trout sequences. Full length cDNA clones encoding ssPOMCA and ssPOMC-B were obtained from a pituitary cDNA library of sockeye salmon using the PCR products as probes. The ssPOMC-A and -B cDNAs have a length of 1072 and 1709 bps, respectively. Northern blot analysis showed that both ssPOMC-A and -B mRNAs were expressed only in the pituitary, and their sizes were about 1.2 kb and 1.8 kb, respectively. The presence of two ssPOMC genes was confirmed by Southern blot analysis of genomic DNA obtained from a single sockeye salmon. The deduced amino acid sequences of the ssPOMC-A and -B contained 230 and 226 residues, respectively. The amino terminal of &bgr;-endorphin in ssPOMC-B which corresponds to Met-enkephalin domain is YSGFM, which is different from YGGFM of Met-enkephalin found in many other vertebrate species. The homology of nucleotide sequences between ssPOMC-A and -B is 59% in the entire coding region, whereas &agr;-MSH coding regions are highly homologous (91 %). Although the deduced amino acid sequences of ssPOMCs show 43% overall similarity, their hydropathy profiles are coincident with those of several other vertebrate species, particularly the amino terminal of N-terminal peptide (NPP) shows almost the same pattern with other vertebrate NPPs.
两栖动物非洲爪蟾中阿片黑皮素原克隆 cDNA 的核苷酸序列。
DOI：
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发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Martens,GJ;Civelli,O;Herbert,E
通讯作者：
Herbert,E
小鼠阿片黑皮质素原的完整氨基酸序列，源自阿片黑皮质素原 cDNA 的核苷酸序列。
DOI：
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发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Uhler,M;Herbert,E
通讯作者：
Herbert,E