A targeted metabolomics approach to characterize acetogenin profiles in avocado fruit (Persea americana Mill.)
A targeted metabolomics approach to characterize acetogenin profiles in avocado fruit (Persea americana Mill.)
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DOI：
10.1039/c5ra22854a
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发表时间：
2015-01-01
期刊：
影响因子：
3.9
通讯作者：
Diaz de la Garza, Rocio I.
中科院分区：
文献类型：
--
作者：
Eduardo Rodriguez-Lopez, Carlos;Hernandez-Brenes, Carmen;Diaz de la Garza, Rocio I.
月桂基乙酸配基是在鳄梨（Persea americana Mill.）中发现的具有奇数碳脂肪族链的脂肪酸衍生物。这些化合物显示出广泛的生物活性，使它们分别在食品工业中用作抗微生物剂和促凋亡剂以及对抗癌细胞。关于它在植物体内代谢的现有知识是稀缺的。这项工作量化了22个鳄梨品种果实组织（果皮，种子和果肉）中积累的8种不同的乙酸配基，使用靶向代谢组学方法对现有的变异进行采样。多变量分析揭示了存在于果实组织和它们的化学骨架中的乙酸配基之间的相关性，该相关性允许将它们分类为三个家族（鳄梨素、Pahuatins和Persenins）的提议。种子的乙酮配基分布不同于果肉和果皮的乙酮配基分布，果肉和果皮的乙酮配基分布虽然浓度不同（果皮积累的乙酮配基量低），但具有相同的分布。来自已知来源的样品中的乙酮配基也使用描述性线性判别分析（LDA）按品种分离，并且通过预测性LDA生成化学分类模型，并对来自未知来源的样品进行测试。这项工作有效地采样了鳄梨果实中种子（1.09-8.33 mg/g FW）、果皮（0.22-12.5 mg/g FW）和果肉（0.49-9.58 mg/g FW）中的乙酮含量和谱变异性，并对7个鳄梨品种进行了推定分类。这项工作的结果表明，在所有22个鳄梨品种中都产生了随后的8种乙酸配基，这表明鳄梨植物之间的保守代谢。
Lauraceous acetogenins are fatty acid derivatives with an odd-carbon aliphatic chain found in avocado (Persea americana Mill.). These compounds display a wide range of bioactivities that makes them candidates for use as antimicrobial and proapoptotic agents in the food industry and against cancer cells respectively. Existing knowledge about its metabolism in planta is scarce. This work quantifies eight different acetogenins accumulated in fruit tissues (peel, seed, and pulp) from 22 avocado cultivars to sample the existing variation using a targeted metabolomics approach. Multivariate analyses uncovered correlations among acetogenins present in fruit tissues and their chemical backbone that allowed a proposal for classifying them in three families (Avocatins, Pahuatins and Persenins). The seed acetogenin profile differed from that of the pulp and peel, which while different in concentration (peel accumulated low acetogenin amounts), had the same profile. Acetogenins from samples of known origin were also separated by variety using descriptive Linear Discriminant Analysis (LDA), and a chemotaxonomic model was generated via predictive LDA and was tested on samples from unknown origin. This work effectively sampled acetogenin contents and profile variability in seed (1.09-8.33 mg per g FW), peel (0.22-12.5 mg per g FW), and pulp (0.49-9.58 mg per g FW) from avocado fruit, as well as provides a putative classification to seven avocado cultivars. Results from this work show that the eight acetogenins followed are produced in all 22 avocado cultivars, which points to conserved metabolism among avocado plants.