Release from quorum-sensing molecules triggers hyphal formation during Candida albicans resumption of growth
Release from quorum-sensing molecules triggers hyphal formation during Candida albicans resumption of growth
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DOI：
10.1128/ec.4.7.1203-1210.2005
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发表时间：
2005-07-01
期刊：
影响因子：
--
通讯作者：
Whiteway, M
中科院分区：
文献类型：
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作者：
Enjalbert, B;Whiteway, M
白色念珠菌是一种病原性真菌，能够随着生长环境的变化而改变形态。简单地将固定细胞接种到37℃的新鲜培养基中，不需要任何其他操作，似乎是一种强大但短暂的菌丝形成诱导剂；这一过程在经典的血清诱导菌丝形成中也发挥着重要作用。其机制似乎涉及到由群体感应分子引起的菌丝抑制在静止相细胞的生长介质中的释放，法尼醇在这一过程中起到了很强但不完全的作用。我们利用DNA微阵列技术研究了白念珠菌细胞的恢复生长和法尼醇对白念珠菌的分子调控。在恢复生长期间保持培养基中的法尼醇既可以延缓和减少菌丝相关基因的诱导，又可以触发编码药物外排组分的基因的表达。法尼醇的存在也阻止了组蛋白基因在菌丝生长过程中的抑制，并影响了可能或已证明具有形态发生调控作用的细胞周期蛋白基因的表达，如HGC1、CLN3和PCL2。这些结果提出了一个基于群体感应分子的模型来解释宿主中菌丝的触发。
Candida albicans is a pathogenic fungus able to change morphology in response to variations in its growth environment. Simple inoculation of stationary cells into fresh medium at 37 degrees C, without any other manipulations, appears to be a powerful but transient inducer of hyphal formation; this process also plays a significant role in classical serum induction of hyphal formation. The mechanism appears to involve the release of hyphal repression caused by quorum-sensing molecules in the growth medium of stationary-phase cells, and farnesol has a strong but incomplete role in this process. We used DNA microarray technology to study both the resumption of growth of Candida albicans cells and molecular regulation involving farnesol. Maintaining farnesol in the culture medium during the resumption of growth both delays and reduces the induction of hypha-related genes yet triggers expression of genes encoding drug efflux components. The persistence of farnesol also prevents the repression of histone genes during hyphal growth and affects the expression of putative or demonstrated morphogenesis-regulating cyclin genes, such as HGC1, CLN3, and PCL2. The results suggest a model explaining the triggering of hyphae in the host based on quorum-sensing molecules.