Revealing the anaerobic acclimation of microbial community in a membrane bioreactor for coking wastewater treatment by Illumina Miseq sequencing
Revealing the anaerobic acclimation of microbial community in a membrane bioreactor for coking wastewater treatment by Illumina Miseq sequencing
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通过 Illumina Miseq 测序揭示焦化废水处理膜生物反应器中微生物群落的厌氧驯化
DOI：
10.1016/j.jes.2017.06.003
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发表时间：
2018
影响因子：
6.9
通讯作者：
Xiaobiao Zhu
中科院分区：
文献类型：
--
作者：
Jiadi Zhu;Lujun Chen;Yan Zhang;Xiaobiao Zhu
采用Illumina Miseq测序法研究了焦化废水MBR处理系统中污泥从好氧驯化到厌氧驯化过程中微生物群落的动态变化。驯化过程中，细菌多样性和微生物多样性均呈先升后降的变化趋势，驯化中期细菌多样性最高。Ignavibacteria变化从极少数（不到1%）的优势菌群（54.0%）随着驯化沿着。β-变形菌的相对丰度相对稳定，在驯化过程中，该类群中某些种类的丰度有所增加，而另一些种类的丰度有所下降。驯化后的厌氧污泥中优势菌群由初始好氧污泥中的嗜盐菌群转变为甲烷菌群。不同驯化阶段的优势菌群和古菌群是初始污泥中的土著微生物，尽管其中一些非常罕见。这一研究支持了“稀有生物圈”中的物种可能最终成为环境变化的主导物种。
The dynamic change of microbial community during sludge acclimation from aerobic to anaerobic in a MBR for coking wastewater treatment was revealed by Illumina Miseq sequencing in this study. The diversity of both Bacteria and Archaea showed an increase–decrease trajectory during acclimation, and exhibited the highest at the domestication interim. Ignavibacteria changed from a tiny minority (less than 1%) to the dominant bacterial group (54.0%) along with acclimation. The relative abundance of Betaproteobacteria kept relatively steady, as in this class some species increased coupled with some other species decreased during acclimation. The dominant Archaea shifted from Halobacteria in initial aerobic sludge to Methanobacteria in the acclimated anaerobic sludge. The dominant bacterial and archaeal groups in different acclimation stages were indigenous microorganisms in the initial sludge, though some of them were very rare. This study supported that the species in “rare biosphere” might eventually become dominant in response to environmental change.