Trichinella spiralis: Synthesis of type IV and type VI collagen during nurse cell formation
Trichinella spiralis: Synthesis of type IV and type VI collagen during nurse cell formation
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DOI：
10.1006/expr.1997.4180
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发表时间：
1997-07-01
影响因子：
2.1
通讯作者：
Despommier, DD
中科院分区：
文献类型：
--
作者：
Polvere, RI;Kabbash, CA;Despommier, DD
含有旋毛虫的骨骼肌纤维（护士细胞）的部分被易被胶原酶消化的无细胞囊包围。抗体识别的IV型和VI型胶原蛋白的胶囊，而高碘酸希夫试剂染色的胶囊差异，揭示了至少两个不同的层。RNA分析表明，特异性的IV型和VI型胶原的mRNA存在于肌肉组织中的第9和15天，但不是在第3天，细胞内感染后。原位杂交显示，这两种类型的mRNA的大部分是在护士细胞，和所有扩大护士细胞核转录活性的这些信息。到第24天，IV型胶原mRNA的合成不存在。相比之下，VI型胶原mRNA在24天和8个月时仍存在。这些结果支持了T. spiralis直接或间接地影响这两种类型的胶原蛋白在其自身发育周期中在护士细胞中的合成。(C)北京：科学出版社.
The portion of skeletal muscle fiber (Nurse cell) harboring Trichinella spiralis is surrounded by an acellular capsule susceptible to digestion with collagenase. Antibodies recognized type IV and type VI collagen in the capsule, while the periodic acid Schiff reagent stained the capsule differentially, revealing at least two distinct layers. RNA analysis showed that mRNA specific for type IV and type VI collagen was present in muscle tissue on Days 9 and 15, but not on Day 3, following intracellular infection. In situ hybridization showed that most of the mRNA for both types was within the Nurse cell, and all enlarged Nurse cell nuclei were transcriptionally active for those messages. Synthesis of type IV collagen mRNA was absent by Day 24. In contrast, type VI collagen mRNA was still present at 24 days and 8 months. These results support the hypothesis that T. spiralis, either directly or indirectly, influences the synthesis of these two collagen types throughout its own developmental cycle in the Nurse cell. (C) 1997 Academic Press.