DETERMINATION OF SECONDARY STRUCTURES OF PROTEINS BY CIRCULAR-DICHROISM AND OPTICAL ROTATORY DISPERSION
DETERMINATION OF SECONDARY STRUCTURES OF PROTEINS BY CIRCULAR-DICHROISM AND OPTICAL ROTATORY DISPERSION
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DOI：
10.1021/bi00772a015
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发表时间：
1972-01-01
期刊：
影响因子：
2.9
通讯作者：
MARTINEZ, HM
中科院分区：
文献类型：
--
作者：
CHEN, YH;YANG, JT;MARTINEZ, HM
陈义雄，|Jen Tsi Yang，* 和Hugo M. Martinez摘要：蛋白质的旋光贡献可以用X=//+ ß ß+/rXr表示，其中X可以是椭圆率或任何波长的旋光。f是螺旋（）、螺旋形式和无序形式（R）的分数; f的和等于1，并且每个f大于或等于零。用X射线衍射研究得到的五种蛋白质的λ值，用最小二乘法拟合了这些蛋白质在任何波长下的λ，λ和λ r。由此确定的螺旋、螺旋和无序形式的圆二色性和旋光色散可以反过来用于估计秒数。
Yee-Hsiung Chen,| Jen Tsi Yang,* and Hugo M. Martinez abstract: The rotatory contributions of a protein can be represented by X=//+ ß ß+/rXr, where X can be either the ellipticity or the rotation at anywavelength. The The f’s are the fractions of the helix (), ß form and unordered form (R); the sum of f’s is equal to unity and each/is greater than or equal to zero. With the/values of five proteins obtained by X-raydiffraction studies, the Y’s of these proteins at anywavelength are fitted by a least-squares method, which defines the Xu, ß, and Xr. The circular dichroism and opti-cal rotatory dispersion for the helix, ß, and unordered form thus determined can be conversely used to estimate the sec-