A role for two-component signaling elements in the Arabidopsis growth recovery response to ethylene
A role for two-component signaling elements in the Arabidopsis growth recovery response to ethylene
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DOI：
10.1002/pld3.58
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发表时间：
2018-05-01
期刊：
影响因子：
3
通讯作者：
Schaller, G. Eric
中科院分区：
文献类型：
--
作者：
Binder, Brad M.;Kim, Hyo Jung;Schaller, G. Eric
以往的研究表明，拟南芥幼苗在乙烯处理后恢复正常生长的能力与乙烯受体的组氨酸激酶活性有关。由于组氨酸激酶可以作为双组分信号系统的输入，我们研究了涉及双组分信号元件的功能丧失突变体。我们发现，磷酸转移蛋白和B型反应调节剂的突变体表现出在其乙烯生长恢复反应的缺陷，发现与功能丧失的乙烯受体突变体etr 1 -7类似。基于对细胞分裂素受体突变体的分析以及对细胞分裂素生物合成的化学抑制，双组分信号元件调节对乙烯功能的生长恢复反应的能力独立于其在细胞分裂素信号传导中的良好表征作用。组氨酸激酶活性的受体ETR 1也有利于生长恢复的乙烯过敏反应，其特征是由一个短暂的下降，生长速度时，幼苗连续处理低剂量的乙烯，然而，这种反应被发现独立的B型反应调节器。这些结果表明，乙烯受体ETR 1的组氨酸激酶活性执行两个独立的功能：（a）通过涉及磷酸转移蛋白和B型反应调节剂的双组分信号系统调节对乙烯的生长恢复，以及（B）以不依赖于B型反应调节剂的方式调节对乙烯的过敏反应。
Previous studies indicate that the ability of Arabidopsis seedlings to recover normal growth following an ethylene treatment involves histidine kinase activity of the ethylene receptors. As histidine kinases can function as inputs for a two-component signaling system, we examined loss-of-function mutants involving two-component signaling elements. We find that mutants of phosphotransfer proteins and type-B response regulators exhibit a defect in their ethylene growth recovery response similar to that found with the loss-of-function ethylene receptor mutant etr1-7. The ability of two-component signaling elements to regulate the growth recovery response to ethylene functions independently from their well-characterized role in cytokinin signaling, based on the analysis of cytokinin receptor mutants as well as following chemical inhibition of cytokinin biosynthesis. Histidine kinase activity of the receptor ETR1 also facilitates growth recovery in the ethylene hypersensitive response, which is characterized by a transient decrease in growth rate when seedlings are treated continuously with a low dose of ethylene; however, this response was found to operate independently of the type-B response regulators. These results indicate that histidine kinase activity of the ethylene receptor ETR1 performs two independent functions: (a) regulating the growth recovery to ethylene through a two-component signaling system involving phosphotransfer proteins and type-B response regulators and (b) regulating the hypersensitive response to ethylene in a type-B response regulator independent manner.