Transcriptomic and metabolomic profiling reveal the mechanism underlying the inhibition of wound healing by ascorbic acid in fresh-cut potato
Transcriptomic and metabolomic profiling reveal the mechanism underlying the inhibition of wound healing by ascorbic acid in fresh-cut potato
复制标题
转录组学和代谢组学分析揭示了鲜切马铃薯中抗坏血酸抑制伤口愈合的机制
DOI：
10.1016/j.foodchem.2023.135444
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发表时间：
2023
期刊：
影响因子：
8.8
通讯作者：
Aili Jiang
中科院分区：
文献类型：
--
作者：
Fuhui Zhou;Dongying Xu;Siguo Xionng;Chen Chen;Chenghui Liu;Aili Jiang
抗坏血酸（阿萨）抑制鲜切马铃薯（FCP）的伤口愈合，然而，在伤口愈合过程中的化学物质的综合调控机制仍不清楚。在这里，进行了生理生物化学，转录组学和代谢组学分析。总共，685差异表达基因（DEG）和1921差异积累的代谢产物（DAMs）之间的控制和砷处理的样品进行了鉴定。在AsA处理的样品中，大多数DEG表达水平和DAMs丰度与新切割样品的数据相似。收集的数据表明，阿萨治疗通过调节谷胱甘肽代谢、增强淀粉代谢和抑制苯丙氨酸代谢、蔗糖降解和脂肪酸合成来抑制FCPs中的伤口愈合。受阿萨处理影响的主要基因和代谢产物包括StGST、StPAL、StPHO1和StLOX 5以及淀粉、蔗糖和亚油酸。阿萨处理提高了淀粉含量、淀粉酶和脂肪氧合酶活性，降低了游离脂肪酸水平。我们的研究为FCP的伤口愈合机制提供了基本的见解。
Ascorbic acid (AsA) inhibits wound healing in fresh-cut potatoes (FCP); however, the comprehensive regulatory mechanisms of the chemical during wound healing remain unclear. Here, physiobiochemical, transcriptomic, and metabolomic analyses were performed. In total, 685 differentially expressed genes (DEGs) and 1921 differentially accumulated metabolites (DAMs) were identified between control and AsA-treated samples. The level of the majority of DEGs expression and DAMs abundance in AsA-treated samples were similar to data of newly cut samples. The collective data indicated that the AsA treatment inhibited wound healing in FCPs by regulating glutathione metabolism, enhancing starch metabolism, and inhibiting phenylalanine metabolism, sucrose degradation, and fatty acid synthesis. Major genes and metabolites affected by AsA treatment includedStGST,StPAL,StPHO1andStLOX5, and starch, sucrose, and linoleic acid. AsA treatment increased starch content and amylase and lipoxygenase activity and decreased free fatty acid level. Our research provides fundamental insights into wound healing mechanisms in FCP.