Quantitative proteomics of rat and human pancreatic beta cells.
Quantitative proteomics of rat and human pancreatic beta cells.
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DOI：
10.1016/j.dib.2015.02.019
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发表时间：
2015-06
期刊：
影响因子：
1.2
通讯作者：
Martens GA
中科院分区：
文献类型：
--
作者：
Brackeva B;Kramer G;Vissers JP;Martens GA
数据集描述：该数据集由无标记交替扫描LC-MS/MS蛋白质组学分析人类和Wistar大鼠胰岛内分泌细胞组成。ProteinLynx GlobalSERVER （PLGS）的人类和大鼠胰腺β细胞的质谱数据以及由此产生的蛋白质组搜索结果已通过PRIDE合作伙伴存储库存入ProteomeXchange Consortium，数据集标识为PXD001539（人类）和PXD001816（大鼠）。从这些质谱数据中，计算出细胞类型和物种之间的“相对摩尔量单位”。生物学相关性：这些数据提供了人类和大鼠β和α细胞未分离蛋白质组的定量观点。它可能偏向于具有较高摩尔丰度的蛋白质，与核心功能途径有关，但也包括几种具有胰岛富集表达的蛋白质。细胞制备的质量是最先进的，无标签定量既精确又准确，允许详细的定量分析。
Data set description: This data set is composed by label-free alternate-scanning LC-MS/MS proteomics analysis human and Wistar rat pancreatic islet endocrine cells. The mass spectrometry data of the human and rat pancreatic beta cells and the resulting proteome search output from ProteinLynx GlobalSERVER (PLGS) have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifiers PXD001539 (human) and PXD001816 (rat). From these mass spectrometry data, ‘relative molar amount units’ between cell types and across species were calculated. Biological relevance: These data provide a quantitative view on the unfractionated proteomes of human and rat beta and alpha cells. It is likely biased towards the proteins with higher molar abundance, relating to core functional pathways, but also includes several proteins with an islet-enriched expression. The quality of the cell preps is state-of-the-art, and the label-free quantitation is both precise and accurate, allowing detailed quantitative analysis.