Fine structure of the subitaneous eggshell of the sessile rotifer Stephanoceros millsii (Monogononta) with observations on vesicle trafficking in the integument during ontogeny

Fine structure of the subitaneous eggshell of the sessile rotifer Stephanoceros millsii (Monogononta) with observations on vesicle trafficking in the integument during ontogeny
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无柄轮虫 Stephanoceros millsii (Monogononta) 下蛋壳的精细结构以及个体发育过程中珠皮内囊泡运输的观察

DOI:
10.1080/07924259.2019.1581097
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发表时间:
2018
影响因子:
0.8
通讯作者:
Wallace, Robert L.
Wallace, Robert L.
中科院分区:
生物学4区
文献类型:
--
作者:
Hochberg, Rick;Yang, Hui;Walsh, Elizabeth J.;Wallace, Robert L.

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参与周期性孤雌生殖的轮虫产生两种类型的卵:作为克隆雌性孵化的皮下卵和作为单倍体雄性孵化的减数分裂卵,或者如果受精,在一段时间滞育后作为雌性孵化(休眠卵)。休眠蛋壳的超微结构在一些活动物种中是已知的,但关于皮下蛋壳的数据有限,没有关于无柄轮虫的任何蛋壳的数据。在这里,我们研究了固着轮虫Stephanoceros Millsii皮下卵壳的超微结构及其潜在的分泌来源、母体卵黄和胚珠被。我们还研究了幼体和成体体壁的分泌活动,以确定在个体发育过程中活动是否发生变化。蛋壳由单层和两个亚层组成:外部颗粒亚层明显来自母体卵黄,内部絮状亚层由胚珠被分泌,可能形成孵化膜或糖萼。幼虫和成虫的分泌活性都很高,似乎是糖萼变厚的来源。总而言之,皮下蛋壳OFS。在单一的轮虫中,毫氏轮虫是最薄的。薄薄的蛋壳可能是对母亲体外试管提供的额外保护做出的反应。
Rotifers that engage in cyclical parthenogenesis produce two types of eggs: subitaneous eggs that hatch as clonal females and meiotic eggs that hatch as haploid males, or if fertilized, as females after a period of diapause (resting eggs). The ultrastructure of resting eggshells is known for some motile species, but there are limited data on subitaneous eggshells, and no data on any eggshells of sessile rotifers. Here, we investigated the ultrastructure of the subitaneous eggshell of the sessile rotiferStephanoceros millsiiand its potential origins of secretion, the maternal vitellarium and embryonic integument. We also explored secretory activity in the larval and adult integuments to determine whether activity changes during ontogeny. The eggshell consists of a single layer with two sublayers: an external granular sublayer apparently derived from the maternal vitellarium, and an internal flocculent sublayer secreted by the embryonic integument that may form a hatching membrane or glycocalyx. Secretory activity remains high in both the larva and adult and appears to be the source of the thickening glycocalyx. Altogether, the subitaneous eggshell ofS. millsiiis the thinnest among monogonont rotifers. Thin eggshells may have evolved in response to the added protection provided by the mother’s extracorporeal tube.
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