Changes of γδT cell subtypes during pregnancy and their influences in spontaneous abortion
Changes of γδT cell subtypes during pregnancy and their influences in spontaneous abortion
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DOI：
10.1016/j.jri.2019.01.003
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发表时间：
2019-02-01
影响因子：
3.4
通讯作者：
Yao, Xiaoying
中科院分区：
文献类型：
--
作者：
Cai, Dongli;Tang, Yunhui;Yao, Xiaoying
成功怀孕是一个复杂的过程，涉及内分泌和免疫系统的精确定时调节。尽管妊娠期免疫学的知识不断增加，但对子宫内膜和蜕膜中免疫细胞的研究仍然是零散的。动态数据缺乏孕前子宫内膜淋巴细胞的过渡到初始妊娠状态以及中期/晚期妊娠状态。在这里，我们确定了γ δ（γ δ）T细胞在子宫内膜和蜕膜的组成，从妇女正常妊娠和原因不明的自然流产。我们发现，γ δ T细胞的频率在怀孕过程中波动，这些变化受到孕酮的调节。与外周血不同，正常妇女子宫内膜和早孕蜕膜中V δ 1（+）γ δ T细胞占绝大多数，子宫内膜间质细胞（ESCs）可能直接参与V δ 1（+）γ δ T细胞的漂移。此外，在早孕蜕膜中以V δ 2亚群为主的γ δ T细胞百分比的增加与原因不明的自然流产有关。我们的研究结果揭示了妊娠期间γ δ T细胞发生的确切时间以及内分泌、免疫细胞与妊娠的密切关系，这将有助于进一步了解和解决不孕症和不明原因自然流产的问题。
A successful pregnancy is a complicated process that involves the precisely timed regulation of endocrine as well as immune system. Despite increasing knowledge about immunology in gestation, the studies of immune cells in endometrium and decidua are still fragmented. Dynamic data is lacking on the transition of pre-pregnancy endometrial lymphocytes to initial pregnancy states as well as to second/third-trimester status. Here, we determined the composition of Gamma delta (gamma delta) T cells in endometrium and decidua from women with normal pregnancy and unexplained spontaneous abortion. We found that the frequency of gamma delta T cells is fluctuating over the course of pregnancy, and these changes were regulated by progesterone. Different from peripheral blood, V delta 1(+) gamma delta T cells accounted for the majority in endometrium and early-pregnancy decidua of healthy women, and endometrial stromal cells (ESCs) may involve in V delta 1(+) gamma delta T shift directly. Moreover, an increase in the percentage of gamma delta T cells with V delta 2 subset predominant in early-pregnancy decidua was associated with unexplained spontaneous abortion. Our results unraveled the precise timing of gamma delta T cells occurring during pregnancy and the close relationship among endocrine, immune cells and pregnancy, which can further help understand and solve the problem of infertility and unexplained spontaneous abortion.