Effect of different selenium sources and levels on porcine circovirus type 2 replication in vitro
Effect of different selenium sources and levels on porcine circovirus type 2 replication in vitro
复制标题
不同硒源和水平对猪圆环病毒2型体外复制的影响
DOI：
10.1016/j.jtemb.2008.02.002
复制
发表时间：
2008-01-01
影响因子：
3.5
通讯作者：
Lu, Fuzeng
中科院分区：
文献类型：
--
作者：
Pan, Qunxing;Huang, Kehe;Lu, Fuzeng
Porcine circovirus type 2 (PCV2) has been linked to several disease syndromes during the last decade. A deficiency in selenium has also been associated with the increases of virulence of some viruses and severity of infectious disease. In order to evaluate the effect of different selenium sources and levels on PCV2 replication in PK-15 cells, three selenium Sources, i.e. sodium selenite, kappa-selenocarrageenan and DL-Selenomethionine at. concentrations of 0, 2, 4, 8, and 16 mu mol/L were used throughout this experiment. PCV2 loads in PK-15 cells were measured by a newly developed real-time quantitative PCR. A significantly inhibitive effect of DL-Selenomethionine on PCV2 replication in vitro was demonstrated and the inhibition was concentration dependent within the range of 2-16 mu mol/L. The inhibitive effect of DL-selenomethionine on PCV2 replication may be caused by enhanced activity of glutathione peroxidase. Out results may serve as a basis for further studies of the biological function of selenium and control of PCV2 infection. (C) 2008 Elsevier GmbH. All rights reserved.
