Mussel-Inspired Biocoating for Improving the Adhesion of Dental Pulp Stem Cells in Dental Pulp Regeneration.
Mussel-Inspired Biocoating for Improving the Adhesion of Dental Pulp Stem Cells in Dental Pulp Regeneration.
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DOI：
10.1002/marc.202000102
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发表时间：
2020-06
影响因子：
4.6
通讯作者：
Yanan Liu;Ying Qiu;S. Ni;Xuewei Zhang;Hongchen Sun;Wenlong Song;Xiangwei Li
中科院分区：
文献类型：
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作者：
Yanan Liu;Ying Qiu;S. Ni;Xuewei Zhang;Hongchen Sun;Wenlong Song;Xiangwei Li
牙髓工程在修复根管内丢失的牙髓和恢复其功能方面具有广阔的应用前景。牙髓干细胞(DPSCs)在牙本质根管壁上的稳定黏附是牙髓再生过程中重建功能性成牙本质细胞层的关键因素。为了应对这一挑战，将多巴胺修饰的透明质酸(DA-HA)包覆在牙本质上，以获得稳定的DPSCs粘附性。多巴胺部分提供涂层的粘附力，透明质酸增加生物相容性。结果表明，DPSCs在DA-HA涂层的牙本质片上的黏附效果好于未涂层的牙本质片。同时，可以进一步促进DPSCs在所制备的涂层上的增殖。因此，DA-HA涂层可能为成牙本质细胞固定于牙本质表面以进行牙髓再生提供了一种可能。
Dental pulp engineering possesses a promising perspective to replacing lost pulp in the root canal and restoring its functions. Stable adhesion of dental pulp stem cells (DPSCs) on the root canal dentin wall is a key element required for reconstruction of a functional odontoblast layer in dental pulp regeneration. To address this challenge, dopamine-modified hyaluronic acid (DA-HA) is coated on dentin to obtain a stable adhesion of DPSCs. The dopamine segment provides adhesion ability to the coating, and the hyaluronic acid increases the biocompatibility. The results show that DPSCs can adhere on the DA-HA coated dentin slice better than those without coating. Simultaneously, DPSCs proliferation can be further promoted on the prepared coating. Therefore, the DA-HA coating may provide a possible way to immobilize odontoblast cell onto dentin surface for pulp regeneration.