Sandwich-format electrochemiluminescence assays for tumor marker based on PAMAM dendrimer-L-cysteine-hollow gold nanosphere nanocomposites.
Sandwich-format electrochemiluminescence assays for tumor marker based on PAMAM dendrimer-L-cysteine-hollow gold nanosphere nanocomposites.
复制标题
DOI：
10.1016/j.bios.2013.10.014
复制
发表时间：
2014-03
影响因子：
12.6
通讯作者：
Ying Zhuo;Guofeng Gui;Y. Chai;Ni Liao;Kai Xiao;R. Yuan
中科院分区：
文献类型：
--
作者：
Ying Zhuo;Guofeng Gui;Y. Chai;Ni Liao;Kai Xiao;R. Yuan
本工作制备了一种新型的聚酰胺-胺（PAMAM）树枝状大分子-半胱氨酸-空心金纳米球纳米复合物，并将其用作过硫酸盐/O2电化学发光体系的促进剂，用于检测肿瘤标志物癌胚抗原（CEA）的浓度。在本文中，羧基封端的PAMAM树枝状聚合物通过EDC/NHS偶联化学用1-半胱氨酸（1-Cys）修饰。然后，以空心金纳米球（HGNP）为载体，通过巯基-Au键合组装PAMAM-l-Cys，并进一步负载检测抗体（Ab 2），形成PAMAM-l-Cys-HGNP-Ab 2生物缀合物。在靶CEA存在下，在沉积的金修饰电极上自组装的捕获抗CEA抗体（Ab 1）与PAMAM-I-Cys-HGNP上的Ab 2之间可以形成夹心免疫结构，从而导致ECL响应的成比例增加，这是由于PAMAM-I-Cys-HGNP对过二硫酸盐/O2 ECL系统的显著增强。结果，开发了用于CEA检测的夹心ECL测定，其具有从20 fg/mL到1.0 ng/mL的大浓度变化的优异灵敏度和6.7 fg mL−1的检测限。
In this work, a novel polyamidoamine (PAMAM) dendrimer-l-cysteine-hollow gold nanospheres nanocomposite was fabricated and used as the promoter for the peroxydisulfate/O2ECL system to detect the concentration of the tumor marker carcinoembryonic antigen (CEA). Herein, the carboxyl-terminated PAMAM dendrimers were decorated withl-cysteine (l-Cys) by EDC/NHS coupling chemistry. Then, the hollow gold nanospheres (HGNPs) were employed as effective nano-carriers for the assembly of PAMAM-l-Cys via thiols-Au bonding, which was used for further loading of detection antibody (Ab2) to form the PAMAM-l-Cys-HGNPs-Ab2bioconjugates. In the presence of target CEA, the sandwiched immuno-structure can be formed between the capture anti-CEA antibodies (Ab1), which self-assembled on deposited gold modified electrode, and the Ab2on the PAMAM-l-Cys-HGNPs, thereby resulting in a proportional increase in ECL response, due to the significant enhancement of PAMAM-l-Cys-HGNPs toward peroxydisulfate/O2ECL system. As a result, a sandwich ECL assay for CEA detection was developed with excellent sensitivity of a large concentration variation from 20 fg/mL to 1.0 ng/mL and a detection limit of 6.7 fg mL−1.