Profiling of bacterial flora in crevices around titanium orthodontic anchor plates
Profiling of bacterial flora in crevices around titanium orthodontic anchor plates
复制标题
DOI：
10.1111/j.1600-0501.2006.01294.x
复制
发表时间：
2007-02-01
影响因子：
4.3
通讯作者：
Takahashi, Nobuhiro
中科院分区：
文献类型：
--
作者：
Sato, Renya;Sato, Takuichi;Takahashi, Nobuhiro
本研究采用厌氧培养和分子生物学技术对正畸钛锚板周围微生物区系进行鉴定，并对17名受试者的钛锚板周围微生物区系和健康龈沟中的微生物组成进行比较对20-29岁的健康人进行厌氧培养，分离出的细菌经16 SrRNA测序鉴定，结果表明：健康人、炎症人和健康锚龈沟中的菌落形成对数单位/ml分别为6.84、7.51和8.88，表明锚板缝隙的细菌密度低于健康牙龈缝隙的细菌密度。从7名受试者的健康锚板缝隙中分离的184株细菌中，108株（59%）为厌氧菌，73株（40%）为兼性细菌。主要分离菌为革兰氏阴性杆菌，如弯曲杆菌（12%）、梭杆菌（10%）和月形单胞菌（10%），以及革兰氏阳性兼性细菌，如放线菌（17%）和链球菌（8.2%）。从3名受试者的发炎锚板缝隙中分离的133株菌株中，110株（83%）为厌氧菌，而主要分离株为革兰氏阴性杆菌，如普雷沃菌属（47%）、梭杆菌属（33%）和弯曲杆菌属（16%）。另一方面，从7名受试者的健康龈沟中分离出的146株细菌中，98株（67%）为兼性细菌，45株（31%）为厌氧菌。结果表明，钛正畸锚板周围的缝隙环境是厌氧的，有利于细菌的厌氧生长，从而可能引发钛正畸锚板周围组织的炎症反应。
The aims of this study were to characterize the microflora in crevices around titanium orthodontic anchor plates using anaerobic culture and molecular biological techniques for bacterial identification, and to compare the microbial composition between crevices around anchor plates and gingival crevices.Samples from crevices around titanium anchor plates and healthy gingival crevices of 17 subjects (aged 20-29) were cultured anaerobically, and isolated bacteria were identified by 16S rRNA sequencing.The average logarithm colony-forming units/ml were 6.84, 7.51 and 8.88 in healthy anchor plate crevices, inflamed anchor plate crevices and healthy gingival crevices, respectively, indicating that the bacterial density of anchor plate crevices was lower than that of healthy gingival crevices. Of 184 strains isolated from healthy anchor plate crevices of seven subjects, 108 (59%) were anaerobic bacteria, while 73 (40%) were facultative bacteria. Predominant isolates were Gram-negative rods, such as Campylobacter (12%), Fusobacterium (10%) and Selenomonas (10%), and Gram-positive facultative bacteria, such as Actinomyces (17%) and Streptococcus (8.2%). Of 133 strains isolated from inflamed anchor plate crevices of three subjects, 110 (83%) were anaerobic bacteria, while predominant isolates were Gram-negative rods, such as Prevotella (47%), Fusobacterium (33%) and Campylobacter (16%). On the other hand, of 146 strains isolated from healthy gingival crevices of seven subjects, 98 (67%) were facultative bacteria, while 45 (31%) were anaerobic bacteria. Predominant isolates were Gram-positive facultative bacteria, such as Actinomyces (37%) and Streptococcus (20%).These results suggest that the environment in crevices around titanium orthodontic anchor plates is anaerobic and supportive of anaerobic growth of bacteria, which may trigger inflammation in the tissue around the plates.