High activated and memory cytotoxic T-cell responses to HTLV-1 in healthy carriers and patients with tropical spastic paraparesis
High activated and memory cytotoxic T-cell responses to HTLV-1 in healthy carriers and patients with tropical spastic paraparesis
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DOI：
10.1006/viro.1996.0101
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发表时间：
1996-03-01
期刊：
影响因子：
3.7
通讯作者：
Bangham, CRM
中科院分区：
文献类型：
--
作者：
Daenke, S;Kermode, AG;Bangham, CRM
对HTLV-1的细胞毒性T淋巴细胞（CTL）应答主要针对Tax蛋白。在大多数健康携带者和HTLV-1相关疾病热带痉挛性轻瘫（HAM/TSP）患者中可以发现循环活化的Tax特异性CTL。在这项研究中，我们提出了26 HTLV-1携带者，包括10个新招募的受试者的Tax特异性CTL反应的数据。在所有研究对象中均未发现Rex特异性CTL。对4例健康携带者、3例HAM/TSP患者和1例血清阴性HAM/TSP患者分别进行了活化和记忆性CTL应答的检测。在所有受试者中，每个表位的肽特异性记忆细胞的平均频率（1/1307）很高。健康携带者和HAM/TSP患者之间每个表位的平均记忆性CTL频率或活化CTL的受试者比例无显著差异。HAM/TSP的一个人有一个异常高的频率响应两个肽，这表明在这个人的表位识别的免疫优势。我们的结论是，HTLV-1特异性CTL反应的幅度和组件之间的健康运营商和HAM/TSP患者没有不同。这些数据不支持HAM/TSP发病机制中的特定CTL介导的组分。(C)出版社：Academic Press
The cytotoxic T-lymphocyte (CTL) response to HTLV-1 is directed mainly against the Tax protein. Circulating, activated Tax-specific CTL can be found in a majority of healthy carriers and patients with the HTLV-1-associated disease tropical spastic paraparesis (HAM/TSP). In this study we present data on the Tax-specific CTL response of 26 HTLV-1 carriers, including 10 newly recruited subjects. Rex-specific CTL were not found in any subjects investigated. Activated and memory CTL responses were determined separately in 4 healthy carriers, 3 HAM/TSP patients, and 1 ''seronegative HAM/TSP.'' In all subjects, the mean frequency of peptide-specific memory cells per epitope (1/1307) was high. There was no significant difference in mean memory CTL frequency per epitope or in the proportion of subjects with activated CTL between healthy carriers and HAM/TSP patients. One individual with HAM/TSP had an unusually high frequency response to two peptides, suggesting immunodominance of epitope recognition in this individual. We conclude that the magnitude and components of the HTLV-1-specific CTL response do not differ between healthy carriers and HAM/TSP patients. These data do not support a specific CTL-mediated component in the pathogenesis of HAM/TSP. (C) 1995 Academic Press, Inc.