Phylum-specific environmental DNA analysis reveals remarkably high global biodiversity of Cercozoa (Protozoa)
Phylum-specific environmental DNA analysis reveals remarkably high global biodiversity of Cercozoa (Protozoa)
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DOI：
10.1099/ijs.0.63229-0
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发表时间：
2004-11-01
影响因子：
2.8
通讯作者：
Cavalier-Smith, T
中科院分区：
文献类型：
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作者：
Bass, D;Cavalier-Smith, T
本研究首次对来自一系列不同栖息地的单一原生动物门（Cercozoa Cavalier-Smith，1998）进行 18S rRNA 多文库环境 PCR 调查。系统发育分析揭示了该门内至少有九个新的进化枝，其中一些可能处于目或更高的水平。描述了进一步的实验，以确定一些进化枝的真实生态和地理分布，这些进化枝可能从树中推断出受到任一或两种方式的限制。这些结果表明，尾兽类群的多样性可能涉及数千个谱系，使其在多样性上可与最大的、特征更清楚的原生动物门相媲美，例如。纤毛虫。 （纤毛虫和吸水虫）和有孔虫。还介绍了培养的海绵单胞菌、Metopiona 和 Metopion 的新序列。鉴于这些补充以及环境图书馆中分类单元采样的增加，对尾兽分类进行了一些修订：将海绵动物门从网纹毛虫转移到单毛毛虫；从肉胞菌门中去除 Metopiida；以及新的Metromonadida目的创建，目前包含Metromonas单一属。尽管Metromonas与Chlorarachnea有弱到中度的支持，但它在这里被放置在超纲Monadofilosa中，它在形态上与后者更相似。
This study presents the first 18S rRNA multi-library environmental PCR survey of a single protozoan phylum, Cercozoa Cavalier-Smith 1998, from a range of different habitats. Phylogenetic analysis reveals at least nine novel clades within the phylum, several possibly at the level of order or above. Further experiments are described to ascertain the true ecological and geographical distributions of some clades that might be inferred from the tree to be restricted in either or both ways. These results suggest that the diversity of cercozoan taxa may run into thousands of lineages, making it comparable in diversity to the largest better-characterized protozoan phyla, e.g. Ciliophora. (ciliates and suctorians) and Foraminifera. New sequences of cultured Spongomonas, Metromonas and Metopion are also presented. In the light of these additions, and the increased taxon sampling from the environmental libraries, some revisions of cercozoan classification are made: the transfer of Spongomonadea from Reticulofilosa to Monadofilosa; the removal of Metopiida from Sarcomonadea; and the creation of the new order Metromonadida, currently containing the single genus Metromonas. Although Metromonas groups with weak to moderate support with Chlorarachnea, it is here placed in superclass Monadofilosa, to which it is morphologically more similar.