Gelsolin regulates proliferation, apoptosis, migration and invasion in human oral carcinoma cells
Gelsolin regulates proliferation, apoptosis, migration and invasion in human oral carcinoma cells
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DOI：
10.3892/ol.2015.3002
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发表时间：
2015-05-01
期刊：
影响因子：
2.9
通讯作者：
Hu, Qingang
中科院分区：
文献类型：
--
作者：
Deng, Runzhi;Hao, Jing;Hu, Qingang
凝溶胶蛋白 (GSN) 是最丰富的肌动蛋白结合蛋白之一，参与多种病理过程，包括阿尔茨海默病、心脏损伤和癌症。本研究的目的是评估 GSN 对口腔鳞状细胞癌 Tca8113 细胞生长和运动的影响。将过表达载体pcDNA3.1-GSN转染Tca8113细胞，并基于G418抗生素选择鉴定出稳定的GSN过表达细胞系。采用细胞计数试剂盒8法、流式细胞仪和Transwell法检测GSN过表达对Tca8113细胞增殖、凋亡、迁移和侵袭的影响。结果显示，GSN过表达显着促进Tca8113细胞的增殖和凋亡。此外，Transwell实验表明GSN过表达增强了Tca8113细胞的迁移和侵袭能力。因此，GSN 的上调可促进细胞生长和运动，表明它可能在人类口腔癌的进展中发挥重要作用。
Gelsolin (GSN) is one of the most abundant actin-binding proteins, and is involved in several pathological processes, including Alzheimer's disease, cardiac injury and cancer. The aim of the present study was to assess the effect of GSN on the growth and motility of oral squamous cell carcinoma Tca8113 cells. The overexpression vector pcDNA3.1-GSN was transfected into Tca8113 cells and the stable GSN overexpression cell line was identified based on G418 antibiotic selection. The effect of GSN overexpression on the proliferation, apoptosis, migration and invasion of Tca8113 cells was examined using a cell counting kit-8 assay, flow cytometry and Transwell assays. The results revealed that GSN overexpression significantly promoted the cell proliferation and apoptosis of Tca8113 cells. In addition, Transwell assays demonstrated that the migration and invasion abilities of Tca8113 cells were enhanced by GSN overexpression. Therefore, the upregulation of GSN promotes cell growth and motility, indicating that it may perform a vital function in the progression of human oral cancers.