The pig as a model for translational research: overview of porcine animal models at Jichi Medical University.
The pig as a model for translational research: overview of porcine animal models at Jichi Medical University.
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DOI：
10.1186/2047-1440-1-8
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发表时间：
2012-08-16
期刊：
影响因子：
--
通讯作者：
Lefor AT
中科院分区：
文献类型：
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作者：
Kobayashi E;Hishikawa S;Teratani T;Lefor AT
为了改善实验动物的福利，研究人员力求尊重 3R 原则（替换、减少和细化）。即使在进行临床试验之前必须进行大型动物研究，寻找减少实验动物数量的方法也很重要。在先进医疗技术发展中心，我们认为“医用”猪是理想的临床前模型系统。我们一直在使用野生猪和转基因猪。大约十年前，我们开始使用这种方法通过“全猪系统”来模拟人类健康和疾病，以达到医疗技能教育以及新设备和治疗策略开发的目的。在我们的中心，医学生和住院医师使用猪来获得手术技能经验，并在适当的模拟训练后进行紧急程序培训。高级临床医生还使用这些模型来推进内窥镜和腹腔镜手术创新工具的开发。该中心专注于器官移植和干细胞治疗的转化研究。已经建立了几种用于肝、肠、肾、胰腺和肺移植的猪模型。间充质基质细胞已在绿色荧光蛋白和红色荧光蛋白转基因猪中建立，并测试其转分化器官发生。我们中心正在进行一项在猪体内建立诱导多能干细胞的计划。在这里，我们回顾了我们在这一领域 10 年的活动。根据我们在外科教育和研究方面的经验，实验猪是转化研究中很有价值的模型。
To improve the welfare of experimental animals, investigators seek to respect the 3R principle (Replacement, Reduction, and Refinement). Even when large animal studies are essential before moving to clinical trials, it is important to look for ways to reduce the number of experimental animals used. At the Center for the Development of Advanced Medical Technology, we consider ‘medical’ pigs to be ideal preclinical model systems. We have been using both wild-type and genetically modified pigs. We began using this approach about 10 years ago with a ‘total pig system’ to model human health and disease for the purposes of both medical skill education and the development of new devices and therapeutic strategies. At our Center, medical students and residents use pigs to gain experience with surgical skills and train for emergency procedures after appropriate simulation training. Senior clinicians have also used these models to advance the development of innovative tools for endo- and laparoscopic procedures. The Center focuses on translational research for organ transplantation and stem cell therapy. Several pig models have been established for liver, intestine, kidney, pancreas, and lung transplantation. Mesenchymal stromal cells have been established in green fluorescent protein- and red fluorescent protein-transgenic pigs and tested to trans-differentiate organogenesis. A program to establish induced pluripotent stem cells in the pig is ongoing at our Center. Here, we review our 10 years of activity in this field. Based on our experience in surgical education and research, experimental pigs are valuable models in translational research.