Voltage Control of Droplet Interface Bilayer Lipid Membrane Dimensions
Voltage Control of Droplet Interface Bilayer Lipid Membrane Dimensions
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DOI：
10.1021/la1036508
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发表时间：
2011-01-18
期刊：
影响因子：
3.9
通讯作者：
Steckl, Andrew J.
中科院分区：
文献类型：
--
作者：
Punnamaraju, Srikoundinya;Steckl, Andrew J.
提出了一种控制无锚点液滴界面双层（DIB）脂膜面积的新方法。在分散于十二烷油中的磷脂包被的水性液滴的界面处形成无支撑的DIB脂质膜。使用插入液滴中的电极，施加调节有效DIB面积的外部电压。同时进行电（电容或电流）和光学（DIB横向长度成像）记录。记录α-溶血素（α HL）单通道插入DIB。测量通过DIB的电流作为电压和液滴中的α HL浓度的函数。观察到电流，DIB横向长度和面积，以及相对于电压的电容的非线性响应。电压引起的界面张力的变化调制DIB-油接触角和膜接触长度，这提供了膜尺寸的控制。这些结果的比较是由电润湿效应，这也是由电压上的界面张力的效果。该方法提供了对插入DIB中的离子通道的数量的主动控制。
A novel approach to control the area of anchor-free droplet interface bilayer (DIB) lipid membranes is presented. Unsupported DIB lipid membranes are formed at the interface of phospholipid-coated aqueous droplets dispensed in dodecane oil. Using electrodes inserted into the droplets, an external voltage is applied which modulates the effective DIB area. Electrical (capacitance or current) and optical (imaging of DIB lateral length) recordings were simultaneously performed. Alpha-hemolysin (alpha HL) single channel insertions into the DIB were recorded. Currents across the DIB were measured as a function of voltage and alpha HL concentration in the droplets. Nonlinear response is observed for current, DIB lateral length and area, and capacitance with respect to voltage. Voltage induced changes in interfacial tension modulated the DIB-oil contact angle and the membrane contact length, which provided control of membrane dimensions. Comparison of these results is made to the electrowetting effect, which is also governed by effect of voltage on the interfacial tension. This approach provides active control of the number of ion channels inserted into the DIB.