Stereological assessment of the total number of hypoglossal neurons after repeated crush injuries to the hypoglossal nerve in adult rats
Stereological assessment of the total number of hypoglossal neurons after repeated crush injuries to the hypoglossal nerve in adult rats
复制标题
成年大鼠舌下神经反复挤压损伤后舌下神经元总数的体视学评估
DOI：
10.1080/01616412.2016.1275461
复制
发表时间：
2017
影响因子：
1.9
通讯作者：
T. Moriizumi
中科院分区：
文献类型：
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作者：
N. Fukushima;M. Karasawa;K. Yokouchi;N. Sumitomo;K. Kawagishi;T. Moriizumi
摘要目的：周围神经轴突损伤后，成熟运动神经元不会发生逆行神经元细胞死亡。然而，以前的研究表明，逆行神经元细胞死亡发生在成年大鼠后，双病变的舌下神经（XII）神经的基础上，在XII神经元的数量大幅减少。使用体视学方法，我们研究了神经细胞凋亡的第十二神经元和第十二神经元的总数后，反复挤压损伤的第十二神经。方法：用脑动脉瘤夹夹闭成年大鼠右侧第十二神经，间隔一周，夹闭三次。在最后一次挤压后4周，从体视学上估计受损右侧和未受损左侧的XII神经元总数。结果：反复挤压损伤后，没有凋亡是明显的，在XII神经元裂解的caspase-3的免疫染色所示。此外，囊泡乙酰胆碱转运蛋白的免疫组化显示，在反复挤压伤后4周的舌头轴突伸长。第4周时，损伤侧和未损伤侧的XII神经元总数分别为7800 ± 290和8000 ± 230，两组间差异无统计学意义。结论：在成年大鼠的XII神经反复挤压损伤后，XII神经元中不发生神经元细胞死亡，XII神经元的总数不减少。
Abstract Objective: Retrograde neuronal cell death does not occur in mature motoneurons following the axonal injury of peripheral nerves. However, a previous study suggested that retrograde neuronal cell death does occur in adult rats after the creation of double lesions on the hypoglossal (XII) nerve based on a substantial decrease in the number of XII neurons. Using stereological methods, we examined neuronal apoptosis in XII neurons and the total number of XII neurons following repeated crush injuries to the XII nerve. Methods: The right XII nerve of adult rats was crushed three times at one-week intervals with a brain aneurysm clip. At 4 weeks after the final crush, the total numbers of XII neurons on the injured right and uninjured left sides were estimated stereologically. Results: After repeated crush injuries, no apoptosis was evident in XII neurons as indicated by immunostaining for cleaved caspase-3. Moreover, immunohistochemistry for the vesicular acetylcholine transporter revealed axonal elongation in the tongue 4 weeks after repeated crush injuries. At 4 weeks, the total numbers of XII neurons were 7800 ± 290 on the injured right side and 8000 ± 230 on the uninjured left side, and no significant difference was evident between the injured and uninjured sides. Conclusion: Neuronal cell death does not occur in XII neurons and the total number of XII neurons does not decrease after repeated crush injuries of the XII nerve in adult rats.
Mitsuyo Maeda、Norihiro Ohba、Saya Nakagomi、Yasuhiro Suzuki、Sumiko Kiryu-Seo、Kazuhiko Namikawa、Wataru Kondoh、Akemi Tanaka、Hiroshi Kiyama：“囊泡乙酰胆碱转运蛋白可以作为再生肌肉神经支配的形态学标记
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