IMMUNOCYTOCHEMICAL LOCALIZATION OF THE CYSTIC-FIBROSIS GENE-PRODUCT CFTR
IMMUNOCYTOCHEMICAL LOCALIZATION OF THE CYSTIC-FIBROSIS GENE-PRODUCT CFTR
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DOI：
10.1073/pnas.88.20.9262
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发表时间：
1991-10-01
影响因子：
11.1
通讯作者：
HIGGINS, CF
中科院分区：
文献类型：
--
作者：
CRAWFORD, I;MALONEY, PC;HIGGINS, CF
针对囊性纤维化基因产物CFTR的不同结构域的两种多肽的抗血清已经被提出，并被广泛地表征。在Western分析中，这两种抗血清都能识别CFTR为165 kDa的多肽，无论是在细胞还是在上皮细胞中。用免疫细胞化学方法研究了CFTR的细胞和组织分布。CFTR在上皮细胞中含量丰富，包括汗管、小胰管和肠腺的衬里细胞。出乎意料的是，CFTR在肺上皮细胞中的表达水平较低，而在肾小管上皮细胞中含量较高。该蛋白似乎仅限于上皮细胞的顶端，而不是基底外侧，至少有一部分与质膜有关。CFTR在细胞和组织中的分布与该蛋白作为氯离子通道或作为通道活性调节器的功能是一致的。
Antisera against two peptides, corresponding to different domains of the cystic fibrosis gene product CFTR, have been raised and extensively characterized. Both antisera recognize CFTR as a 165-kDa polypeptide in Western analysis of cells transfected with CFTR cDNA as well as in epithelial cell lines. The cell and tissue distribution of CFTR has been studied by immunocytochemistry. CFTR is abundant in epithelial cells, including those lining sweat ducts, small pancreatic ducts, and intestinal crypts. Unexpectedly, the level of CFTR in lung epithelia is relatively low, while it is abundant in the epithelia of kidney tubules. The protein appears to be restricted to the apical, rather than basolateral, regions of epithelial cells and at least a proportion is associated with the plasma membrane. The cell and tissue distributions of CFTR are consistent with a function for this protein as a chloride channel or as a regulator of channel activity.