Anatomical Methods to Study the Suprachiasmatic Nucleus.
Anatomical Methods to Study the Suprachiasmatic Nucleus.
复制标题
研究视交叉上核的解剖方法。
DOI：
10.1007/978-1-0716-2249-0_13
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Bittman,EricL
中科院分区：
文献类型：
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作者：
Bittman,EricL
哺乳动物视交叉上核(SCN)起着主要的昼夜节律起搏器的作用。为了研究它保持时间、携带周期性环境信号(沸石)以及调节大脑其他地方和外围的从属振荡器的机制，人们应用了各种分子方法。多标记免疫细胞化学和原位杂交提供了对广泛用于研究SCN的生理学方法(如体外电生理学和发光法)的补充。解剖学方法需要解释从不同时间点处死的一组单独动物收集的数据。这对实验的设计施加了限制，这些实验旨在观察自由运行条件下昼夜节律的变化。在这样的实验中，解释受试者不同时期的差异是至关重要的。然而，作为神经调节剂或神经递质的重要转录本和/或其多肽产物的细胞内共定位和区域表达是可能的。有了这些工具和其他工具，对下丘脑起搏器调节昼夜节律功能的机制的了解正在快速发展。
The mammalian suprachiasmatic nucleus (SCN) functions as a master circadian pacemaker. In order to examine mechanisms by which it keeps time, entrains to periodic environmental signals (zeitgebers), and regulates subordinate oscillators elsewhere in the brain and in the periphery, a variety of molecular methods have been applied. Multiple label immunocytochemistry and in situ hybridization provide anatomical insights that complement physiological approaches (such as ex vivo electrophysiology and luminometry) widely used to study the SCN.The anatomical methods require interpretation of data gathered from groups of individual animals sacrificed at different time points. This imposes constraints on the design of the experiments that aim to observe changes that occur with circadian phase in free-running conditions. It is essential in such experiments to account for differences in the periods of the subjects. Nevertheless, it is possible to resolve intracellular colocalization and regional expression of functionally important transcripts and/or their peptide products that serve as neuromodulators or neurotransmitters. Armed with these tools and others, understanding of the mechanisms by which the hypothalamic pacemaker regulates circadian function is progressing apace.