Tangentially Migrating Neurons Assemble a Primary Cilium that Promotes Their Reorientation to the Cortical Plate
Tangentially Migrating Neurons Assemble a Primary Cilium that Promotes Their Reorientation to the Cortical Plate
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DOI：
10.1016/j.neuron.2012.10.027
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发表时间：
2012-12-20
期刊：
影响因子：
16.2
通讯作者：
Metin, Christine
中科院分区：
文献类型：
--
作者：
Baudoin, Jean-Pierre;Viou, Lucie;Metin, Christine
在迁移的神经元中，中心体形成并固定了一个极化的微管网络，指导细胞器的运动。我们在这里报道，从内侧神经节隆起（MGE）切向迁移的神经元的母中心粒组装了一个短的初级纤毛，并在引导过程中通过与质膜对接将纤毛暴露在细胞表面。初级纤毛是由鞭毛内运输（IFT）形成的，这也是脊椎动物Sonic hedgehog （Shh）信号转导所必需的。我们发现Shh通路的扰动影响了MGE细胞的主要过程形态和动力学。Shh有利于MGE细胞在发育中的皮层中离开切向迁移路径，而环巴胺或IFT基因的失效使MGE细胞保持在切向迁移路径上。我们的研究结果表明，通过初级纤毛传递的信号促进了未来gaba能中间神经元从切向途径逃逸到皮层板上定居。
In migrating neurons, the centrosome nucleates and anchors a polarized network of microtubules that directs organelle movements. We report here that the mother centriole of neurons migrating tangentially from the medial ganglionic eminence (MGE) assembles a short primary cilium and exposes this cilium to the cell surface by docking to the plasma membrane in the leading process. Primary cilia are built by intraflagellar transport (IFT), which is also required for Sonic hedgehog (Shh) signal transduction in vertebrates. We show that Shh pathway perturbations influenced the leading process morphology and dynamics of MGE cells. Whereas Shh favored the exit of MGE cells away from their tangential migratory paths in the developing cortex, cyclopamine or invalidation of IFT genes maintained MGE cells in the tangential paths. Our findings show that signals transmitted through the primary cilium promote the escape of future GABAergic interneurons from their tangential routes to colonize the cortical plate.