Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.)
Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.)
复制标题
DOI：
10.1007/bf02172406
复制
发表时间：
1996-10
期刊：
影响因子：
--
通讯作者：
O. Panaud;X. Chen;S. McCouch
中科院分区：
文献类型：
--
作者：
O. Panaud;X. Chen;S. McCouch
含有简单重复序列（SSR）的微卫星标记是一种很有价值的遗传分析工具。我们的目的是扩大现有的水稻RFLP图谱与简单序列长度多态性（SSLP）。在这项研究中，我们描述了20个新的微卫星标记，已被分配到位置沿着水稻染色体，其特点是在栽培稻和野生稻的等位基因多样性，并测试扩增在远亲物种。我们的研究结果表明，微卫星在水稻基因组中的分布似乎是随机的，没有明显的偏见，或在特定区域的聚类，定位结果是相同的亚种间和种间群体，野生近缘种的扩增是可靠的栽培稻，但变得不太成功的遗传距离增加。SSLP等位基因序列分析表明，在一个单一的300 bp的区域内，每个独立的变异的复杂阵列的SSR基序的聚类。两个微卫星标记扩增多个位点，被定位到独立的水稻染色体上，这表明在水稻基因组中存在重复的区域。随着SSLP标记数量的增加，水稻基因组分析的能力和分辨率有望得到提高。
Microsatellite markers containing simple sequence repeats (SSR) are a valuable tool for genetic analysis. Our objective is to augment the existing RFLP map of rice with simple sequence length polymorphisms (SSLP). In this study, we describe 20 new microsatellite markers that have been assigned to positions along the rice chromosomes, characterized for their allelic diversity in cultivated and wild rice, and tested for amplification in distantly related species. Our results indicate that the genomic distribution of microsatellites in rice appears to be random, with no obvious bias for, or clustering in particular regions, that mapping results are identical in intersubspecific and interspecific populations, and that amplification in wild relatives ofOryza sativais reliable in species most closely related to cultivated rice but becomes less successful as the genetic distance increases. Sequence analysis of SSLP alleles in three relatedindicavarieties demonstrated the clustering of complex arrays of SSR motifs in a single 300-bp region with independent variation in each. Two microsatellite markers amplified multiple loci that were mapped onto independent rice chromosomes, suggesting the presence of duplicated regions within the rice genome. The availability of increasing numbers of mapped SSLP markers can be expected to increase the power and resolution of genome analysis in rice.