MICROTUBULE SURFACE LATTICE AND SUBUNIT STRUCTURE AND OBSERVATIONS ON REASSEMBLY
MICROTUBULE SURFACE LATTICE AND SUBUNIT STRUCTURE AND OBSERVATIONS ON REASSEMBLY
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DOI：
10.1083/jcb.60.1.153
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发表时间：
1974-01-01
影响因子：
7.8
通讯作者：
ERICKSON, HP
中科院分区：
文献类型：
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作者：
ERICKSON, HP
神经元微管由脑组织匀浆重新组装并纯化。在从纯化的制剂中重新组装时，形成的第一个结构是平板，由多达 13 根纵向细丝组成，被鉴定为不完整的微管壁。通过光学衍射分析这些平板和完整微管的电子显微照片，确定亚基排列的表面晶格为13丝、3起始螺旋。外层双联微管也发现了类似且可能相同的晶格。最后，通过用计算机滤波和平均系统处理选定的图像，获得微管亚基的结构和排列的二维图像。 40 x 50 Å 的形态亚基以前仅被视为球状颗粒，被鉴定为 55,000 道尔顿的微管蛋白单体，在这张高分辨率的重建图像中可以看到被拉长，并通过纵向裂缝对称地分裂成两个叶。
Neuronal microtubules have been reassembled from brain tissue homogenates and purified. In reassembly from purified preparations, one of the first structures formed was a flat sheet, consisting of up to 13 longitudinal filaments, which was identified as an incomplete microtubule wall. Electron micrographs of these flat sheets and intact microtubules were analyzed by optical diffraction, and the surface lattice on which the subunits are arranged was determined to be a 13 filament, 3-start helix. A similar, and probably identical, lattice was found for outer-doublet microtubules. Finally, a 2-D image of the structure and arrangement of the microtubule subunits was obtained by processing selected images with a computer filtering and averaging system. The 40 x 50 Å morphological subunit, which has previously been seen only as a globular particle and identified as the 55,000-dalton tubulin monomer, is seen in this higher resolution reconstructed image to be elongated, and split symmetrically by a longitudinal cleft into two lobes.