Single-walled carbon nanotubes can induce pulmonary injury in mouse model
Single-walled carbon nanotubes can induce pulmonary injury in mouse model
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DOI：
10.1021/nl0723634
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发表时间：
2008-02-01
期刊：
影响因子：
10.8
通讯作者：
Yang, Pan-Chyr
中科院分区：
文献类型：
--
作者：
Chou, Cheng-Chung;Hsiao, Hsiang-Yun;Yang, Pan-Chyr
碳纳米管是一种广泛应用于工业的纳米材料。碳纳米管长期暴露的潜在健康风险已引起公众的极大关注。在本研究中，我们已经证明，intrichiheal滴注0.5毫克的单壁碳纳米管（SWCNT）到雄性ICR小鼠（8周龄）诱导肺泡巨噬细胞活化，各种慢性炎症反应，和严重的肺肉芽肿形成。然后，我们使用Affyphon微阵列来研究暴露于SWCNT时对巨噬细胞的分子效应。生物学途径分析、文献调查和实验验证表明，巨噬细胞摄取SWCNT能够激活各种转录因子，如核因子κ B（NF-κ B）和激活蛋白1（AP-1），这导致氧化应激、促炎细胞因子的释放、白细胞的募集、保护性和抗凋亡基因表达的诱导，和T细胞的激活。由此产生的先天性和适应性免疫应答可以解释SWCNT引起的慢性肺部炎症和肉芽肿形成。
Carbon nanotubes are a nanomaterial that is extensively used in industry. The potential health risk of chronic carbon nanotubes exposure has been raised as of great public concern. In the present study, we have demonstrated that intratracheal instillation of 0.5 mg of single-walled carbon nanotubes (SWCNT) into male ICR mice (8 weeks old) induced alveolar macrophage activation, various chronic inflammatory responses, and severe pulmonary granuloma formation. We then used Affymetrix microarrays to investigate the molecular effects on the macrophages when exposed to SWCNT. A biological pathway analysis, a literature survey, and experimental validation suggest that the uptake of SWCNT into the macrophages is able to activate various transcription factors such as nuclear factor kappa B (NF-kappa B) and activator protein 1 (AP-1), and this leads to oxidative stress, the release of proinflammatory cytokines, the recruitment of leukocytes, the induction of protective and antiapoptotic gene expression, and the activation of T cells. The resulting innate and adaptive immune responses may explain the chronic pulmonary inflammation and granuloma formation in vivo caused by SWCNT.