QUANTITATION OF THE 3 NORMALLY OCCURRING PLASMA FIBRINOGENS IN HEALTH AND DURING SOCALLED ACUTE PHASE BY SDS ELECTROPHORESIS OF FIBRIN OBTAINED FROM EDTA-PLASMA
QUANTITATION OF THE 3 NORMALLY OCCURRING PLASMA FIBRINOGENS IN HEALTH AND DURING SOCALLED ACUTE PHASE BY SDS ELECTROPHORESIS OF FIBRIN OBTAINED FROM EDTA-PLASMA
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DOI：
10.1016/0049-3848(84)90359-1
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发表时间：
1984-01-01
影响因子：
7.5
通讯作者：
GODAL, HC
中科院分区：
文献类型：
--
作者：
HOLM, B;GODAL, HC
正常人血浆含有不同分子量的纤维蛋白原。为了定量这些纤维蛋白原，在8 mM EDTA存在下，在pH 6.4下用7.5NIH [National Institutes of Health] U/ml凝血酶使血浆凝结。将凝块溶解在6.6 M尿素中，并在含有3%聚丙烯酰胺/0.5%琼脂糖的凝胶上进行SDS [十二烷基硫酸钠]电泳，并通过光密度扫描对组分进行定量。该方法重复性好，变异系数为1.5%。发现三种主要纤维蛋白原级分：高分子量纤维蛋白原（HMW，MW 340，000）、低分子量纤维蛋白原（LMW，MW 300，000）和LMW“（MW 280，000）。此外，还可观察到5条弱带。在123名年龄1-93岁的男性和女性健康受试者的血浆中，HMW为69.7 ±。5.1%，低分子量物质26.5 ±。4.8%，LMW为3.8 ±。占总纤维蛋白原的1.8%。女性和老年受试者的HMW相对量显示出较小但具有统计学显著性的降低（2-4%）。手术和广泛急性心肌梗死后，HMW/LMW比值发生变化。定期记录的总纤维蛋白原的大幅增加主要是由于HMW在3-4天后达到最大值。LMW在前2天保持不变，然后略有增加，最大值延迟（8-11天）。
Normal human plasma contains fibrinogens of different MW. To quantitate these fibrinogens, plasma was clotted at pH 6.4 with 7.5 NIH [National Institutes of Health] U/ml thrombin in the presence of 8 mM EDTA. The clots were dissolved in 6.6 M urea and submitted to SDS [sodium dodecyl sulfate] electrophoresis on gels containing 3% polyacrylamide/0.5% agarose, and the fractions quantitated by densitometric scanning. The reproducibility of this method was high with variation coefficient 1.5%. Three main fibrinogen fractions were found: high MW fibrinogen (HMW, MW 340,000), low MW fibrinogen (LMW, MW 300,O00) and LMW'' (MW 280,000). In addition, 5 weak bands could be seen. In plasma from 123 healthy subjects of both sexes, aged 1-93 yr, HMW constituted 69.7 .+-. 5.1%, LMW 26.5 .+-. 4.8% and LMW'' 3.8 .+-. 1.8% of the total fibrinogen. Women and older subjects displayed a small, but statistically significant reduction in the relative amounts of HMW (2-4%). Following surgery and extensive acute myocardial infarction the HMW/LMW ratio changed. The substantial increase in total fibrinogen regularly recorded was mainly due to HMW that reached maximal values after 3-4 days. LMW remained unchanged the first 2 days and then displayed a slight increase with a delayed maximum (8-11 days).