Performance of new clean-up column for the determination of ochratoxin A in cereals and foodstuffs by HPLC-FLD
Performance of new clean-up column for the determination of ochratoxin A in cereals and foodstuffs by HPLC-FLD
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DOI：
10.1080/02652030400023143
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发表时间：
2004-11-01
影响因子：
2.9
通讯作者：
Krska, R
中科院分区：
文献类型：
--
作者：
Buttinger, G;Fuchs, E;Krska, R
对新开发的Mycoep((R))229 Ochra和Multisep((R))229 Ochra洗脱柱的性能进行了评价。采用反相高效液相色谱-荧光检测法测定OTA的含量。对谷物、红酒、葡萄干和生咖啡等经常受到污染的商品的回收率进行了估计。小麦的回收率为87.9+/-12.5%(n=6)，葡萄干的回收率为99.4+/-2.7%(n=24)。对于Multisep 229 Ochra，回收率从大麦的76.5+/-8.0%(n=6)提高到葡萄干的86.4+/-1.4%(n=24)。所研究的所有基质(玉米、小麦、大米、大麦、葡萄干、青咖啡豆、红酒)的检出限在0.4-2.4毫克公斤(-1)的范围内。使用经过认证的标准物质对方法的真实性进行了测试。
The performance of the newly developed Mycosep((R)) 229 Ochra and Multisep((R)) 229 Ochra clean-up columns for ochratoxin A (OTA) determination was evaluated. OTA was subsequently analysed using RP-HPLC with fluorescence detection. Recoveries for frequently contaminated commodities, like cereals, red wine, raisins and green coffee, were estimated. The recoveries obtained for the Mycosep 229 Ochra column were in the range from 87.9 +/- 12.5% (n = 6) for wheat to 99.4 +/- 2.7% (n = 24) for raisins. For Multisep 229 Ochra, recoveries from 76.5 +/- 8.0% (n = 6) for barley to 86.4 +/- 1.4% (n = 24) for raisins were achieved. Limits of detection for all matrices investigated (maize, wheat, rice, barley, raisins, green coffee beans, red wine) were in the range 0.4-2.4 mug kg(-1). The trueness of the method was tested using a certified reference material.