Induction of endostatin expression in meniscal fibrochondrocytes by co-culture with endothelial cells
Induction of endostatin expression in meniscal fibrochondrocytes by co-culture with endothelial cells
复制标题
DOI：
10.1007/s00402-008-0766-8
复制
发表时间：
2009-08-01
影响因子：
2.3
通讯作者：
Rudert, Maximilian
中科院分区：
文献类型：
--
作者：
Hoberg, Maik;Schmidt, Edda L.;Rudert, Maximilian
半月板损伤后的最终目标是保留原始半月板组织，但由于半月板损伤（尤其是无血管区）的愈合能力有限，这通常是不可能的。血管内皮细胞产生的因子可能有助于血管化区域的伤口愈合。因此，我们研究了不同的血管生成因子、生长激素和细胞因子在人纤维软骨细胞中以及在与内皮细胞共培养后的纤维软骨细胞中的表达，以更详细地检查尿道损伤的修复机制，并研究内皮细胞在共培养中用于利用组织工程技术进行自体尿道修复的潜在用途。MMP-1，MMP-3，MMP-13，聚集蛋白聚糖，双糖蛋白聚糖，波形蛋白，胶原蛋白-I，-II，-III，-IV，-VI，-X，-XVIII，血管生成素-1，血管生成素-2，和血小板生成抑制素-1在纤维软骨细胞中进行了研究，并与人脐静脉内皮细胞（HUVEC）共培养的细胞进行了比较。在细胞上清液中计数内皮抑制素的表达。在HUVEC存在的情况下，椎间盘纤维软骨细胞表达SMAD-4、iNOS、IL-1 β、VEGF、MMP-1、MMP-3、MMP-13、聚集蛋白聚糖、双糖蛋白聚糖、波形蛋白、胶原-I、-II、-III、-VI和-XVIII，其速率与没有HUVEC的细胞相当。值得注意的是，内皮抑制素的表达显着较高的共培养时相比，单独的纤维软骨细胞cultures和内皮细胞的增殖率显着降低culture.We的结论是，抗血管生成因子内皮抑制素的表达增加的纤维软骨细胞。这可能会限制体内神经损伤的再生能力。
The ultimate goal after meniscus damage is the preservation of the original meniscal tissue, which is often impossible due to the limited healing capacity of meniscal lesions, especially in the avascular zone. Factors produced by endothelial cells of meniscal vessels may contribute to better wound healing in vascularized zones. We therefore investigated the expression of different angiogenic factors, growth hormones and cytokines in human fibrochondrocytes and in fibrochondrocytes upon co-culture with endothelial cells, to examine mechanisms of repair of meniscal injury in more detail and to investigate the potential use of endothelial cells in co-cultures for autologous meniscal repair utilizing tissue engineering technology.Gene expression of SMAD-4, iNOS, IL-1 beta, VEGF, MMP-1, MMP-3, MMP-13, aggrecan, biglycan, vimentin, collagen-I, -II, -III, -IV, -VI, -X, -XVIII, angiopoietin-1, angiopoietin-2, and thrombostatin-1 were investigated in fibrochondrocytes in comparison to cells in co-culture with human umbilical vein endothelial cells (HUVEC). The expression of endostatin was enumerated in cell supernatants. A proliferation assay was used to investigate the mitotic activity of the cells.In presence of HUVEC, meniscal fibrochondrocytes expressed SMAD-4, iNOS, IL-1 beta, VEGF, MMP-1, MMP-3, MMP-13, aggrecan, biglycan, vimentin, collagen-I, -II, -III, -VI, and -XVIII at rates comparable to cells without HUVECs. Note that the expression of endostatin was significantly higher in the co-culture when compared to the separate fibrochondrocyte cultures and the proliferation rate of endothelial cells was significantly decreased in co-culture.We conclude that the expression of the anti-angiogenic factor endostatin increased in the fibrochondrocytes. This may limit the regeneration capacities of meniscal injury in vivo.