MRI monitoring of neuroinflammation in mouse focal ischemia
MRI monitoring of neuroinflammation in mouse focal ischemia
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DOI：
10.1161/01.str.0000252159.05702.00
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发表时间：
2007-01-01
期刊：
影响因子：
8.3
通讯作者：
Berthezene, Yves
中科院分区：
文献类型：
--
作者：
Wiart, Marlene;Davoust, Nathalie;Berthezene, Yves
背景和目的-越来越多的证据表明炎症过程参与了卒中的病理生理过程。吞噬细胞，包括常驻小胶质细胞和浸润性巨噬细胞，分泌保护性和毒性分子，因此代表了潜在的治疗靶点。本研究的目的是监测吞噬细胞的活动后，局灶性脑缺血mice. Methods-Ultrasmall超顺磁性氧化铁颗粒（USPIO）静脉注射后，永久性大脑中动脉闭塞和高分辨率MRI监测72 hours.Results-We在这里提出的第一个MRI数据显示在体内吞噬细胞标记获得小鼠局灶性脑缺血。USPIO增强MRI动力学分析揭示了缺血性病变周围和对侧半球通过胼胝体的炎症反应。在伤后36小时内收集的成像数据表明，USPIO相关信号从同侧半球扩散到对侧半球。成像数据与组织化学分析显示炎症远离病变和摄取的纳米颗粒的小胶质细胞/macrophages.Conclusions-The本研究表明，磁共振跟踪的吞噬细胞在小鼠中是可行的，这可能具有重要的治疗意义的潜在的神经毒性激活的小胶质细胞/巨噬细胞在中枢神经系统疾病。
Background and Purpose-A growing body of evidence suggests that inflammatory processes are involved in the pathophysiology of stroke. Phagocyte cells, involving resident microglia and infiltrating macrophages, secrete both protective and toxic molecules and thus represent a potential therapeutic target. The aim of the present study was to monitor phagocytic activity after focal cerebral ischemia in mice.Methods-Ultrasmall superparamagnetic particles of iron oxide (USPIO) were intravenously injected after permanent middle cerebral artery occlusion and monitored by high resolution MRI for 72 hours.Results-We here present the first MRI data showing in vivo phagocyte-labeling obtained in mice with focal cerebral ischemia. USPIO-enhanced MRI kinetic analysis disclosed an inflammatory response surrounding the ischemic lesion and in the contralateral hemisphere via the corpus callosum. The imaging data collected during the first 36 hours postinjury suggested a spread of USPIO-related signal from ipsi- to contralateral hemisphere. Imaging data correlated with histochemical analysis showing inflammation remote from the lesion and ingestion of nanoparticles by microglia/macrophages.Conclusions-The present study shows that MR-tracking of phagocyte cells is feasible in mice, which may have critical therapeutic implications given the potential neurotoxicity of activated microglia/macrophages in central nervous system disorders.