ORIGIN OF THE ELECTRORETINOGRAM IN THE INTACT MACAQUE EYE .1. PRINCIPAL COMPONENT ANALYSIS
ORIGIN OF THE ELECTRORETINOGRAM IN THE INTACT MACAQUE EYE .1. PRINCIPAL COMPONENT ANALYSIS
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DOI：
10.1016/0042-6989(85)90176-2
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发表时间：
1985-01-01
期刊：
影响因子：
1.8
通讯作者：
VANNORREN, D
中科院分区：
文献类型：
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作者：
HEYNEN, H;VANNORREN, D
在完整的猕猴眼睛视网膜的不同深度记录了400毫秒白光闪烁的反应。采用主成分分析（PCA）统计技术，利用细胞对LERG的响应随视网膜深度的变化贡献，从LERG（局部视网膜电图）中分离出独立成分。评估了两种方法：第一，对完整的LERG波形进行PCA，第二，对LERG的小部分进行PCA。第一种方法产生了一个非常类似于受体反应的成分，除了在近端层有一些不一致，以及一个以b波为最突出特征的成分，但仍然包含一些其他反应。这些成分不适合从特定细胞类型的反应来描述LERG。第二种方法使用PCA来确定小时间窗口内的潜在变化是否由单个分量决定。这种方法基本上利用了不同细胞类型响应不同延迟时间的知识，产生了3个组件。这些被鉴定为受体成分，b波和直流成分。评估了这些元件的电压-深度分布。
Responses to 400 ms flashes of white light were recorded at various depths in the retina of the intact macaque eye. A statistical technique, principal component analysis (PCA), was used to isolate independent components from the LERG [local electroretinogram], using the changing contribution of a cell''s response to the LERG with retinal depth. Two approaches were evaluated: 1st, PCA was performed on the complete LERG waveforms and, 2nd, on small sections of the LERG. The 1st method yielded a component strongly resembling the receptor response, apart from some inconsistencies in the proximal layers, and a component in which the b-wave was the most prominent feature, but which still contained some other responses. The components were not suitable to describe the LERG in terms of responses of specific cell types. The 2nd method uses PCA to determine whether or not the potential change within a small time window is accounted for by a single component. This method, which essentially uses the knowledge that different cell types respond with different delay times, yielded 3 components. These were identified as the receptor component, the b-wave and the d.c.-component. The voltage-depth profiles of these components were assessed.