FlbC is a putative nuclear C2H2 transcription factor regulating development in Aspergillus nidulans
FlbC is a putative nuclear C2H2 transcription factor regulating development in Aspergillus nidulans
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DOI：
10.1111/j.1365-2958.2010.07282.x
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发表时间：
2010-09-01
影响因子：
3.6
通讯作者：
Yu, Jae-Hyuk
中科院分区：
文献类型：
--
作者：
Kwon, Nak-Jung;Garzia, Aitor;Yu, Jae-Hyuk
曲霉中的无性发育（分生物）受多个调节剂的约束。在这里，我们表征了曲曲霉中的上游开发激活剂FLBC。在整个生命周期中，在营养生长，早期的无性和晚期性发育阶段，FLBC mRNA可检测到相对较高的水平。 FLBC的缺失会导致分散，BRLA和VOSA表达以及分生孢子发芽的延迟/减少。虽然FLBC（OEFLBC）的过表达并未详细说明分生孢子，但它抑制了菌丝生长并激活BRLA，ABAA和VOSA的表达，而不是WETA。 FLBC在丝状comcycetes中保守，其中包含两个C端的C2H2锌指和N末端的假定激活结构域。 FLBC定位在菌丝和发育细胞的核中。 FLBC的定位和表达不受FLBB或FLBE的缺乏的影响，反之亦然。重要的是，在不存在BRLA和ABAA的情况下，FLBC的过表达会导致ABAA和VOSA的生长抑制和激活。体外DNA结合测定法表明，FLBC与BRLA，ABAA和VOSA结合，而不是WETA，启动子。总而言之，FLBC是适当激活分生物所必需的推定核转录因子，其平衡活性对于尼古拉（A. nidulans）的生长和发展至关重要。
P>Asexual development (conidiation) in Aspergillus is governed by multiple regulators. Here, we characterize the upstream developmental activator FlbC in Aspergillus nidulans. flbC mRNA is detectable throughout the life cycle, at relatively high levels during vegetative growth, early asexual and late sexual developmental phases. The deletion of flbC causes a delay/reduction in conidiation, brlA and vosA expression, and conidial germination. While overexpression of flbC (OEflbC) does not elaborate conidiophores, it inhibits hyphal growth and activates expression of brlA, abaA and vosA, but not wetA. FlbC is conserved in filamentous Ascomycetes containing two C2H2 zinc fingers at the C-terminus and a putative activation domain at the N-terminus. FlbC localizes in the nuclei of both hyphae and developmental cells. Localization and expression of FlbC are not affected by the absence of FlbB or FlbE, and vice versa. Importantly, overexpression of flbC causes growth inhibition and activation of abaA and vosA in the absence of brlA and abaA respectively. In vitro DNA-binding assay reveals that FlbC binds to the brlA, abaA and vosA, but not the wetA, promoters. In summary, FlbC is a putative nuclear transcription factor necessary for proper activation of conidiation, and its balanced activity is crucial for governing growth and development in A. nidulans.