A new human neuroblastoma cell line: Biological characteristics, cytogenetics and N-myc oncogene analysis.
A new human neuroblastoma cell line: Biological characteristics, cytogenetics and N-myc oncogene analysis.
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一种新的人神经母细胞瘤细胞系：生物学特性、细胞遗传学和 N-myc 癌基因分析。
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
F. Saito
中科院分区：
文献类型：
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作者：
Toshimitsu Suzuki;M. Hirota;M. Iwabuchi;N. Nomura;F. Saito
用裸鼠连续异种移植的人神经母细胞瘤建立了一个新的人神经母细胞瘤细胞系NB-39-nu。该细胞系是潜在的肾上腺素能，因为腹腔接种NB-39-nu到无胸腺小鼠产生了玫瑰花结-星形细胞型的神经母细胞瘤与酪氨酸羟化酶免疫反应。群体倍增时间在MEM培养基中为41小时，在RPMI-1640培养基中为22小时。众数为83。核型分析显示#1和#2染色体的结构畸变，并且均匀染色区域插入到#10染色体的长臂内。超微结构上，该细胞系主要为未成熟的神经母细胞，但也有少量中分化的神经母细胞。二丁酰环磷酸腺苷，视黄酸和霍乱毒素，但没有神经生长因子诱导的神经突起生长，没有任何可检测的生化分化。癌基因分析显示N-myc DNA扩增和mRNA过表达。N-myc mRNA的水平，然而，没有改变后的细胞系与二丁酰环AMP或视黄酸处理。
A new human neuroblastoma cell line designated NB-39-nu has been established from a human neuroblastoma serialy heterotransplanted in athymic nude mice. The cell line was potentially adrenergic, since intraperitoneal inoculation of NB-39-nu into athymic mice produced a neuroblastoma of rosette-fibrillary type with tyrosine hydroxylaseimmunoreactivity. Population doubling time was 41 hrs in the MEM medium and 22 hrs in the RPMI-1640 medium. Modal chromosome number was 83. Karyotypic analysis revealed the structural aberrations of #1 and #2 chromosomes and homogeneously staining regions were inserted within the long arm of chromosome # 10. Ultrastructurally the cell line was mainly immature neuroblastic cells but a few of moderately differentiated neuroblastic cells were also included. Dibutyryl cyclic AMP, retinoic acid and cholera toxin but not nerve growth factor induced outgrowth of neurites without any detectable biochemical differentiation. Oncogene analysis indicated amplification of N-myc DNA and over-expression of its mRNA. The level of N-myc mRNA, however, did not change after the treatment of the cell line with dibutyryl cyclic AMP or retinoic acid.
缺乏 N-myc 基因扩增的人神经母细胞瘤细胞系的表征。
DOI：
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发表时间：
1988
期刊：
Progress in clinical and biological research
影响因子：
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作者：
Wada,RK;Seeger,RC;Brodeur,GM;Slamon,DJ;Rayner,SA;Tomayko,M;Reynolds,CP
通讯作者：
Reynolds,CP
具有一个或多个癌基因拷贝的神经母细胞瘤表达 N-myc。
DOI：
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发表时间：
1988
期刊：
Progress in clinical and biological research
影响因子：
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作者：
Seeger,RC;Wada,R;Brodeur,GM;Moss,TJ;Bjork,RL;Sousa,L;Slamon,DJ
通讯作者：
Slamon,DJ