Evaluation of a modified interferon-gamma release assay for the diagnosis of latent tuberculosis infection in adult and paediatric populations that enables delayed processing.
Evaluation of a modified interferon-gamma release assay for the diagnosis of latent tuberculosis infection in adult and paediatric populations that enables delayed processing.
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DOI：
10.3109/00365548.2010.498021
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发表时间：
2010-12
影响因子：
--
通讯作者：
Turner J
中科院分区：
文献类型：
--
作者：
Wang SH;Powell DA;Nagaraja HN;Morris JD;Schlesinger LS;Turner J
The objective of the study was to evaluate the specificity of a modified interferon gamma release assay procedure that allows storage of blood samples for up to 32 hours before processing. A total of 116 subjects were enrolled for the study. Two blood samples were collected from each volunteer; one specimen was processed within 8 hours and analyzed using the T-SPOT.TB test; the second specimen was stored overnight and processed 23 to 32 hours later after addition of the T-Cell Xtend™ reagent and then analyzed using the T-SPOT.TB test. A total of 108 paired T-SPOT.TB and T-SPOT.TB plus T-Cell Xtend™ tests were analyzed on specimens from 97 adults and 11 children. The median age of the subjects was 28 years old with 68.5% female and 78.7% White. The overall agreement between the two tests was 98.2% (106/108). Specificity of the T-SPOT.TB test was 99.1% (107/108) and for T-SPOT.TB plus T-Cell Xtend was 97.2%. The two tests were comparable in results. Increasing storage time of the collected blood specimen prior to processing provides flexibility for clinicians and laboratories. Additional studies in larger and diverse patient populations including immunocompromised, pediatric, patients with active TB disease or latent tuberculosis infection are needed.
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DOI：
10.1164/rccm.200701-057oc
发表时间：
2008-02-01
影响因子：
24.7
作者：
Bennett, Diane E.;Courval, Jeanne M.;Castro, Kenneth G.
通讯作者：
Castro, Kenneth G.
影响因子：
3.7
作者：
Smith SG;Joosten SA;Verscheure V;Pathan AA;McShane H;Ottenhoff TH;Dockrell HM;Mascart F
通讯作者：
Mascart F
影响因子：
158.5
作者：
Horsburgh, CR
通讯作者：
Horsburgh, CR
影响因子：
28.2
作者：
Lenders, L. M.;Meldau, R.;Dheda, K.
通讯作者：
Dheda, K.