Structure, Functional Expression, and Cerebral Localization of the Levocabastine-Sensitive Neurotensin/Neuromedin N Receptor from Mouse Brain
Structure, Functional Expression, and Cerebral Localization of the Levocabastine-Sensitive Neurotensin/Neuromedin N Receptor from Mouse Brain
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DOI：
10.1523/jneurosci.16-18-05613.1996
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发表时间：
1996-09
期刊：
影响因子：
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通讯作者：
J. Mazella;J. Botto;E. Guillemare;T. Coppola;P. Sarret;J. Vincent
中科院分区：
文献类型：
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作者：
J. Mazella;J. Botto;E. Guillemare;T. Coppola;P. Sarret;J. Vincent
本工作描述了小鼠脑中左卡巴斯汀敏感性神经降压素（NT）受体的克隆和表达。该受体蛋白包含417个氨基酸，具有G蛋白偶联受体的特征。这种新的NT受体（NTR）类型与迄今为止从大鼠脑和人HT 29细胞系克隆的唯一其他NTR有39%的同源性，但几乎完全不同。当受体在非洲爪蟾卵母细胞中表达时，H1抗组胺药物左卡巴斯汀，如NT和神经介肽N，触发内向电流。这种受体的药理学性质对应于那些低亲和力，左卡巴斯汀敏感的NT结合位点最初在大鼠和小鼠脑制备的膜中描述。在成年小鼠大脑的小脑、海马、梨状皮质和新皮质中表达最多。
This work describes the cloning and expression of the levocabastine-sensitive neurotensin (NT) receptor from mouse brain. The receptor protein comprises 417 amino acids and bears the characteristics of G-protein-coupled receptors. This new NT receptor (NTR) type is 39% homologous to, but pharmacologically distinct from, the only other NTR cloned to date from the rat brain and the human HT29 cell line. When the receptor is expressed in Xenopus laevis oocytes, the H1 antihistaminic drug levocabastine, like NT and neuromedin N, triggers an inward current. The pharmacological properties of this receptor correspond to those of the low-affinity, levocabastine-sensitive NT binding site described initially in membranes prepared from rat and mouse brain. It is expressed maximally in the cerebellum, hippocampus, piriform cortex, and neocortex of adult mouse brain.