Effect of human serum on the electrical detection of amyloid-β fibrils in biological environments using azo-dye immobilized field effect transistor (FET) biosensor
Effect of human serum on the electrical detection of amyloid-β fibrils in biological environments using azo-dye immobilized field effect transistor (FET) biosensor
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DOI：
10.1016/j.sbsr.2018.01.003
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发表时间：
2018-02-01
影响因子：
5.3
通讯作者：
Osaka, Tetsuya
中科院分区：
文献类型：
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作者：
Hideshima, Sho;Wustoni, Shofarul;Osaka, Tetsuya
由于淀粉样β多肽1-42(Aβ42)被发现是阿尔茨海默病的一个新的重要生物标志物，检测人血清等生物样本中的Aβ42对于评估潜在的疾病状态和确定合适的治疗方法具有非常重要的意义。在这项研究中，我们开发了一种基于场效应晶体管(FET)生物传感器的电子分析策略，作为一种简单而可靠的技术来确认生物样品中是否存在Aβ42聚集体(原纤维)。通过将刚果红固定在FET栅极表面作为生物识别元件，我们观察到了检测Aβ42纤维的显著敏感性和特异性。此外，我们还对程序进行了优化，以最大限度地减少大量人血清白蛋白对HS样品检测系统的干扰。优化的刚果红固定FET系统可以在100-PM水平上测量HS样品中的Aβ42纤维，低于其临床浓度。FET设备可用作生物传感系统，用于大量和常规筛选与阿尔茨海默病相关的多肽生物标记物。
As amyloid-beta peptide 1-42 (A beta 42) was found to be an emerging and important biomarker for Alzheimer's disease, the detection of this peptide in biological samples such as human serum (HS) has become very important for evaluating the potential disease state and determining the appropriate treatment. In this study, we developed an electrical analysis strategy based on a field effect transistor (FET) biosensor as a simple and reliable technique for confirming the presence of A beta 42 aggregates (fibrils) in biological samples. By utilizing Congo red immobilized on the FET gate surface as a biorecognition element, we observed remarkable sensitivity and specificity for detecting A beta 42 fibrils. Furthermore, we optimized the procedure to minimize the interference of abundant human serum albumin for the detection system using HS samples. The optimized system of Congo red-immobilized FET enables measurement of A beta 42 fibrils in the 100-pM level in HS samples, which is lower than its clinical concentration. The FET device can be applied as a biosensing system for mass and routine screening of peptide biomarkers related to Alzheimer's disease.