Distribution of freed-serine in vertebrate brains
Distribution of freed-serine in vertebrate brains
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DOI：
10.1016/0006-8993(94)91932-1
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发表时间：
1994-01
期刊：
影响因子：
2.9
通讯作者：
Y. Nagata;K. Horiike;T. Maeda
中科院分区：
文献类型：
--
作者：
Y. Nagata;K. Horiike;T. Maeda
研究了游离丝氨酸在脊椎动物脑中的分布。在较低的脊椎动物物种，鲤鱼，青蛙和鸡的大脑区域，游离丝氨酸水平低。相反，在哺乳动物（小鼠、大鼠和牛）中，前脑中游离丝氨酸含量高（约400 nmol/g湿重，d-丝氨酸与丝氨酸的比值为d/l= 0.4），后脑中含量低。在发育中的小鼠，d-丝氨酸在大脑中的水平随着年龄的增长而增加，并在出生后8周达到成人水平（d/l= 0.40）。在小脑和脑干中，游离丝氨酸水平随着年龄的增加而增加，直到2周龄，然后下降到成人水平：thed/l比值保持不变，直到2周龄，然后下降到0.03，在小脑和脑干中的0.12。使用无菌小鼠显示游离丝氨酸不是微生物来源的。在大鼠前脑中，d-丝氨酸均匀分布在两个大脑区域，即额叶和枕叶。前脑其他区域（海马和下丘脑）的d/l值与大脑相当（d/l= 4），嗅球的d/l值较低（d/l= 0.12）。大鼠大脑灰质d-丝氨酸含量显著高于白色质。牛大脑和小脑中游离丝氨酸的含量与其它哺乳动物相似，但牛大脑灰质中游离丝氨酸/L的比值低于大脑白色质。d-丝氨酸水平的d-氨基酸氧化酶的活性进行了讨论。
Freed-serine distribution in vertebrate brains was investigated. In various brain regions of the lower vertebrate species, carp, frog and chick, freed-serine levels were low. On the contrary, in the mammals, mouse, rat and bull, the contents of freed-serine were high in the forebrain (around 400 nmol/g wet weight, and the ratio ofd-serine tol-serine, wasd/l= 0.4), and low in the hindbrain. In developing mice,d-serine levels in the cerebrum increased with age and attained the adult level (d/l= 0.40) 8 weeks after birth. In the cerebellum and brain stem, the freed-serine levels increased with age until 2 weeks, followed by a decrease to the adult levels: thed/lratios remained constant until 2 weeks of age, then decreased to 0.03 in the cerebellum and 0.12 in the brain stem. Freed-serine was shown not to be of microbial origin using germ-free mice. In the rat forebrain,d-serine was evenly distributed in two cerebral regions, namely frontal and occipital lobes. Thed/lratios in other regions of forebrain, hippocampus and hypothalamus, were comparable to the cerebrum (d/l= 4), while that in the olfactory bulb was lower (d/l= 0.12). In the rat cerebrum, thed-serine content in the grey matter was significantly higher than that in the white matter. The contents of freed-serine in bovine cerebrum and cerebellum were similar to those in other mammalian brains, but thed/lratio for bovine cerebral grey matter was lower than that for the cerebral white matter. Thed-serine level was discussed in terms ofd-amino-acid oxidase activity.