Community structure of culturable bacteria on surface of Gulkana Glacier, Alaska
Community structure of culturable bacteria on surface of Gulkana Glacier, Alaska
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DOI：
10.1016/j.polar.2010.12.002
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发表时间：
2011-04
期刊：
影响因子：
1.8
通讯作者：
T. Segawa;Yoshitaka Yoshimura;Kenichi Watanabe;H. Kanda;S. Kohshima
中科院分区：
文献类型：
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作者：
T. Segawa;Yoshitaka Yoshimura;Kenichi Watanabe;H. Kanda;S. Kohshima
采用培养法对阿拉斯加Gulkana冰川表面的活菌群落进行了研究。分别使用R2A、稀释R2A （DR2A）、LB、稀释LB （DLB）和木糖琼脂在4、15和25℃条件下分离活菌。在DR2A琼脂板上，在4°C时观察到的菌落形成单位（CFU）数量最高，范围为104-105CFU mL−1。共分离得到234株形态不同的菌株。冰川冰雪样品以betaproteobacteria（97株）和gammaproteobacteria（87株）为主。通过扩增菌株的16S rRNA基因对细菌群落进行检测，得到34个类群，类群相似性为bb0 ~ 99%。在这些种型中，26种（76.5%）与以前在寒冷环境中记录的细菌中发现的种型相似。其中5个种型出现在先前独立培养研究的克隆文库中，对应于克隆文库中的6.5%。我们的研究结果表明，寒冷环境中有常见的可培养细菌，这可能会使我们更好地了解冰川上的细菌多样性，并结合分子研究。
Viable bacterial communities on the surface of Gulkana Glacier (Alaska) were investigated using a cultivation method. Viable bacteria were isolated using R2A, diluted-R2A (DR2A), LB, diluted-LB (DLB), and xylose agar at 4, 15 and 25 °C. The highest number of colony-forming units (CFU) was observed on DR2A agar plates at 4 °C, ranging from 104–105CFU mL−1. A collection of 234 morphologically distinct isolates was obtained in total. The glacial snow and ice sample was dominated byBetaproteobacteria(97 isolates) andGammaproteobacteria(87 isolates). The bacterial communities were examined by amplifying 16S rRNA genes from the isolates, and 34 phylotypes with >99% similarities were obtained. Of these phylotypes, 26 (76.5%) were similar to those phylotypes found in bacteria that were previously recorded from cold environments. Five of the phylotypes appeared in the clone library of a previous independent cultivation study, corresponding with 6.5% in the clone library. Our results suggest that cold environments harbor common phylotypes of culturable bacteria, which could possibly lead to a better understanding of bacterial diversity on glaciers in combination with molecular studies.