Targeted tissue oxidation in the cerebral cortex induces local prolonged depolarization and cortical spreading depression in the rat brain
Targeted tissue oxidation in the cerebral cortex induces local prolonged depolarization and cortical spreading depression in the rat brain
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DOI：
10.1016/s0006-291x(02)02906-6
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发表时间：
2003-01-17
影响因子：
3.1
通讯作者：
Watanabe, Y
中科院分区：
文献类型：
--
作者：
Cui, Y;Kataoka, Y;Watanabe, Y
扩散性抑郁症（SD）与癫痫、偏头痛先兆、创伤和脑缺血等神经系统疾病有关，这些疾病也受到脑氧化还原稳态紊乱的影响。为了研究局部组织氧化是否直接诱导SD，我们使用光动力组织氧化（PDTO）技术氧化大鼠大脑皮层的限制性局部区域，并检查氧化区域内和周围的脑血流量（CBF）和直流电（DC）电位。高强度的PDTO仅在光氧化区引起长时间的去极化，导致CBF和DC电位的整体变化：DC电位同步负移（幅度约为20 mV）和CBF过度灌注。DC电位和CBF的变化以约3 mm/min的速率扩散到氧化区域以外的整个大脑皮层半球，表明强烈的局部氧化诱导大鼠脑中的SD。(C)2002 Elsevier Science（美国）。All rights reserved.
Spreading depression (SD) has been linked to several neurological disorders as epilepsy, migraine aura, trauma, and cerebral ischemia, which were also influenced by disorderliness of the brain redox homeostasis. To investigate whether local tissue oxidation directly induces SD, we oxidized a restricted local area of the rat cerebral cortex using photo-dynamic tissue oxidation (PDTO) technique and examined the cerebral blood flow (CBF) and direct current (DC) potential in and around the oxidized area. Intensive PDTO induced prolonged depolarization only in the photo-oxidized area, which led to global changes of CBF and DC potential: synchronous negative shifts of DC potential (with an amplitude of approximately 20 mV) and hyperperfusion of CBF occurred. The changes in DC potential and CBF spread at a rate of around 3 mm/min beyond the oxidized area to the whole hemisphere of the cerebral cortex, indicating that intensive local oxidation induces SD in the rat brain. (C) 2002 Elsevier Science (USA). All rights reserved.