Optical micromanipulation of nanoparticles and cells inside living zebrafish.
Optical micromanipulation of nanoparticles and cells inside living zebrafish.
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DOI：
10.1038/ncomms10974
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发表时间：
2016-03-21
影响因子：
16.6
通讯作者：
Koster G
中科院分区：
文献类型：
--
作者：
Johansen PL;Fenaroli F;Evensen L;Griffiths G;Koster G
生物过程的调节通常基于细胞与其微环境之间的物理相互作用。为了揭示相互作用如何以及在何处发生，可以使用显微操作方法，对相互作用的持续时间，位置和幅度进行高精度控制。然而，由于缺乏体内系统，显微操作通常是在体外对细胞进行的，这可能无法反映多细胞生物体内的复杂情况。在这里，使用光学镊子，我们展示了整个透明的斑马鱼胚胎显微操作。我们表明，不同的细胞，以及注射的纳米粒子和细菌可以被捕获，并可以分析内皮细胞和巨噬细胞的粘附特性和膜变形。这种在整个生物体内的非侵入性显微操作可以直接了解使用现有方法无法访问的细胞相互作用。潜在的应用包括筛选用于癌症治疗或癌症和感染生物学中的组织侵入研究的纳米颗粒-细胞相互作用。 操纵细胞与其环境的相互作用通常是在体外用细胞完成的，这并不反映复杂的体内系统。在这里，作者演示了使用光学镊子对活体斑马鱼体内的微粒、细菌和免疫细胞进行显微操作。
Regulation of biological processes is often based on physical interactions between cells and their microenvironment. To unravel how and where interactions occur, micromanipulation methods can be used that offer high-precision control over the duration, position and magnitude of interactions. However, lacking an in vivo system, micromanipulation has generally been done with cells in vitro, which may not reflect the complex in vivo situation inside multicellular organisms. Here using optical tweezers we demonstrate micromanipulation throughout the transparent zebrafish embryo. We show that different cells, as well as injected nanoparticles and bacteria can be trapped and that adhesion properties and membrane deformation of endothelium and macrophages can be analysed. This non-invasive micromanipulation inside a whole-organism gives direct insights into cell interactions that are not accessible using existing approaches. Potential applications include screening of nanoparticle-cell interactions for cancer therapy or tissue invasion studies in cancer and infection biology. Manipulating the interactions of cells with their environment is usually done with cells in vitro, which does not reflect the complex in vivo system. Here the authors demonstrate micromanipulation of microparticles, bacteria and immune cells within live zebrafish using optical tweezers.