Identification and developmental expression of the ets gene family in the sea urchin (Strongylocentrotus purpuratus)
Identification and developmental expression of the ets gene family in the sea urchin (Strongylocentrotus purpuratus)
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DOI：
10.1016/j.ydbio.2006.08.012
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发表时间：
2006-12-01
影响因子：
2.7
通讯作者：
Arnone, Maria I.
中科院分区：
文献类型：
--
作者：
Rizzo, Francesca;Fernandez-Serra, Montserrat;Arnone, Maria I.
对紫球海胆基因组可用骨架的系统搜索显示，这种海胆有11个ets基因家族成员。对这些基因的系统发育分析表明，几乎所有的脊椎动物ets亚科，除了一个迄今为止只在哺乳动物中发现的外，每个亚科都有一个直齿海胆基因。在胚胎发生过程中的时间和空间表达的ETS因素进行了分析。5个ets基因（Sp-Ets 1/2、Sp-Tel、Sp-Pea、Sp-Ets 4、Sp-Erf）也在母体表达。三个基因（Sp-Elk，Sp-Elf，Sp-Erf）在胚胎发生过程中普遍表达，而另外两个基因（Sp-Gabp，Sp-Pu.1）直到幼虫晚期才转录。值得注意的是，在胚胎发生期间表达的9个海胆ets基因中的5个在间充质细胞和/或其祖细胞中专门（Sp-Ets 1/2、Sp-Erg、Sp-Ese）或另外（Sp-Tel、Sp-Pea）表达。Sp-Ets 1/2的功能分析先前已经证明了该基因在骨骼生成间充质谱系的规范中的重要作用。动态的，在某些情况下，重叠和/或独特的，后五个基因的发育表达模式表明一个复杂的，非冗余的功能ETS因素在海胆间充质形成和分化。(c)2006年爱思唯尔公司All rights reserved.
A systematic search in the available scaffolds of the Strongylocentrotus purpuratus genome has revealed that this sea urchin has 11 members of the ets gene family. A phylogenetic analysis of these genes showed that almost all vertebrate ets subfamilies, with the exception of one, so far found only in mammals, are each represented by one orthologous sea urchin gene. The temporal and spatial expression of the identified ETS factors was also analyzed during embryogenesis. Five ets genes (Sp-Ets1/2, Sp-Tel, Sp-Pea, Sp-Ets4, Sp-Erf) are also maternally expressed. Three genes (Sp-Elk, Sp-Elf, Sp-Erf) are ubiquitously expressed during embryogenesis, while two others (Sp-Gabp, Sp-Pu.1) are not transcribed until late larval stages. Remarkably, five of the nine sea urchin ets genes expressed during embryogenesis are exclusively (Sp-Ets1/2, Sp-Erg, Sp-Ese) or additionally (Sp-Tel, Sp-Pea) expressed in mesenchyme cells and/or their progenitors. Functional analysis of Sp-Ets1/2 has previously demonstrated an essential role of this gene in the specification of the skeletogenic mesenchyme lineage. The dynamic, and in some cases overlapping and/or unique, developmental expression pattern of the latter five genes suggests a complex, non-redundant function for ETS factors in sea urchin mesenchyme formation and differentiation. (c) 2006 Elsevier Inc. All rights reserved.